The Effect of Wild Leek (Allium Ampeloprasum) on Growth and Survival of Lactobacillus Acidophilus and Sensory Properties in Iranian White Cheese

Document Type : Original Paper

Authors

1 Assistant Professor, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 MS Graduated of Microbial Biotechnology, Urmia Branch, Islamic Azad University, Urmia, Iran

3 PhD Student of Food Hygiene, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

The survival ability of probiotic bacteria in food products is one of the most important challenges ahead. The wild leek (Allium ampeloprasum) herb of the Allium family contains various prebiotic compounds that can stimulate the growth of probiotic bacteria. In this research, sensory properties of cheese based on Allium ampeloprasum as a medicinal plant and flavoring was evaluated. For this purpose, after chemical tests on raw milk, different cheese treatments were prepared to determine the effect of fresh and dry concentrations of 1 and 2% of plants, as well as non-herb control containing probiotic bacteria Lactobacillus acidophilus (PTCC 1643) on the growth of Lactobacillus acidophilus bacteria. The results showed that probiotic bacteria Lactobacillus acidophilus had decreasing trend in all treatments and control during storage, but in the treatments containing Allium ampeloprasum, this trend was less. At the end of the 45th day, the lowest bacterial count (Log CFU/g) was observed in the treatment without plant (6.69) and the highest in the sample containing one percent dry plant (8.12). The results of pH assessment also showed that in all samples, the process of pH reduction observed naturally with time of the cheese ripening. However, in treatments containing plant, there was significant difference between 30 and 45 days with non- plant control (P≤0.05). At the end of the 45th day, the lowest bacterial count (Log CFU/g) was observed in the treatment without plant (6.69) and the highest in the sample containing one percent dry plant (8.12). In sensory evaluation, samples containing 1% dry plant and probiotic bacteria had the highest score among different treatments and specified the addition of Allium ampeloprasum as a plant additive could increase the sensory properties of the product and could be successfully used to produce synbiotic cheese.

Keywords

Akın, N., Aydemir, S., Koçak, C., & Yıldız, M.A. (2003). Changes of free fatty acid contents and sensory properties of white pickled cheese during ripening. Food Chemistry, 80(1), 77-83. doi: https://doi.org/10.1016/S0308-8146(02)00242-X
Araújo, E.A., de Carvalho, A.F., dos Santos Leandro, E., de Moraes, C.A., & Furtado, M.M. (2009). Production of cottage-like symbiotic cheese and study of probiotic cells survival when exposed to different stress. Pesquisa Agropecuária Tropical, 39(2), 111-118.
Azambuja, N.C., Zacarchenco, P.B., Fleuri, L.F., Andrade, J.C., Moreno, I., Van Dender, A.G.F., & Gallina, D.A. (2013). Characterization of fresh cheese with addition of probiotics and prebiotics. Journal of Life Sciences, 7(2), 189-195.
Bergamini, C.V., Hynes, E.R., Quiberoni, A., Suarez, V.B., & Zalazar, C.A. (2005). Probiotic bacteria as adjunct starters: influence of the addition methodology on their survival in a semi-hard Argentinean cheese. Food Research International, 38(5), 597-604. doi: https://doi.org/10.1016/j.foodres.2004.11.013
Bernaert, N. (2013). Bioactive compounds in leek (Allium ampeloprasum var. porrum): analysis as a function of the genetic diversity, harvest time and processing techniques (Doctoral dissertation, Ghent University).
Boylston, T.D., Vinderola, C.G., Ghoddusi, H.B., & Reinheimer, J.A. (2004). Incorporation of bifidobacteria into cheeses: challenges and rewards. International Dairy Journal, 14(5), 375-387. doi: https://doi.org/10.1016/j.idairyj.2003.08.008
Buriti, F.C., Okazaki, T.Y., Alegro, J.H., & Saad, S.M. (2007). Effect of a probiotic mixed culture on texture profile and sensory performance of Mines fresh cheese in comparison with the traditional products. Archivos Latinoamericanos De Nutricion, 57(2), 179-185.
China, R., Mukherjee, S., Sen, S., Bose, S., Datta, S., Koley, H., Ghosh, S., & Dhar, P. (2012). Antimicrobial activity of sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus. Microbiological Research, 167(8), 500-506. doi: https://doi.org/10.1016/j.micres.2012.04.003
Çelik, S.E., Özyürek, M., Altun, M., Bektaşoğlu, B., Güçlü, K., Berker, K.I., Ozgokce, F., & Apak, R. (2008). Antioxidant capacities of herbal plants used in the manufacture of Van herby cheese: Otlu peynir. International Journal of Food Properties, 11(4), 747-761. doi: https://doi.org/10.1080/10942910701594210
Dehnavi, F., Khosrowshahi-Asl, A., & Zomorodi Sh. (2013). Viability of lactobacillus acidophilus and its effect on characteristics of jug cheese (technical note). Journal of Agricultural Engineering Research, 14(3), 113-120. (in Persian)
El-Khalek, A.B.A., El-Sayed, H.S., Ibrahim, G.A., El-Shafei, K., El-Din, H.M., Sharaf, O.M., Tawfek, N.F., Effat, B.A., & El-Messery, T.M. (2016). Phenolic compounds, Microbial content and Sensory evaluation of Synbiotic labneh containing Ginger and Probiotic. International Journal of ChemTech Research, 9(2), 238-247.
Fritsch, R.M., &Keusgen, M. (2006). Occurrence and taxonomic significance of cysteine sulphoxides in the genus allium l. (alliaceae). Phytochemistry, 67(11), 1127-1135. doi: https://doi.org/10.1016/j.phytochem.2006.03.006
Ghaemi, H., Hesari, J., & Pourahmad, R. (2010). Production of synbiotic UF Iranian white cheese using lactobacillus acidophilus and inulin. Electronic Journal of Food Processing and Preservation, 4(8), 19-32. (in Persian)
Hap, S., & Gutierrez, N.A. (2012). Functional properties of some New Zealand fruit extracts towards selected probiotic and pathogenic bacteria. Beneficial Microbes, 3(4), 309-318. doi: https://doi.org/10.3920/BM2012.0004
Hui, Y.H. (1993). Dairy science and technology handbook. (Vol. 1). Applications Science, Technology, and Engineering. Wiley-VCH Verlag GmbH.
IDF. (1997). Sensory evaluation of dairy products by scoring. (IDF Standard 99C). Brussels: International Dairy Federation
Iranian National Standardization Organization. (2002a). Determination of milk fat (ISIRI Standard No. 760). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=13487 (in Persian)
Iranian National Standardization Organization. (2002b). Determination of milk protein (ISIRI Standard No. 639). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=4399 (in Persian)
Iranian National Standardization Organization. (2001). Milk and milk product, determination of titrable acidity and value pH-test method, (ISIRI Standard No. 2852). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=34479 (in Persian)
Iranian National Standardization Organization. (2011). Brine and fresh white cheese-Code of practice for production, (ISIRI Standard No. 5772). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=35929 (in Persian)
Isolauri E. (2004). The role of probiotics in pediatrics. Current Pediatrics, 14(2), 104-109. doi: https://doi.org/10.1016/j.cupe.2003.11.002
Joint FAO/WHO. (2002). WHO Working group report on drafting guidelines for the evaluation of probiotics in food. London, Ontario, Canada, April 30 and May 1.
Kasimoglu, A., Goncuoglu, M., & Akgun, S. (2004). Probiotic white cheese with Lactobacillus acidophilus. International Dairy Journal, 14(12), 1067-1073. doi: https://doi.org/10.1016/j.idairyj.2004.04.006
Krasaekoopt, W., Bhandari, B., & Deeth, H. (2004). The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. International Dairy Journal, 14(8), 737-743. doi: https://doi.org/10.1016/j.idairyj.2004.01.004
Kumari, K., & Augusti, K.T. (2002). Antidiabetic and antioxidant effects of S-methyl cysteine sulfoxide isolated from onions (allium cepa linn) as compared to standard drugs in alloxan diabetic rats. Indian Journal of Experimental Biology, 40, 1005-1009.
Marhamatizadeh, M.H., Rezazadeh, S., Najafzadeh, N., & Shahbazi, A. (2009). Studying on survival lactobacillus acidophilus and bifidobacterium bifidum microbes and count them in probiotical milk and yoghurt. Veterinary Research (Garmsar Branch), 5(1), 47-51. (in Persian)
Masihinejad, A., Javadi, M., Barikani, A., Mazloomi, S.M., & Qajarbeigi, P. (2014). Impact of prangos ferulacea on some microbial, physicochemical and sensory properties of probiotic low fat yogurt containing lactobacillus casei. Journal of health sciences and surveillance system, 2(4), 158-163.
Mazinani, S., Fadaie, V., & Khosravi-Darani, K. (2015). Viability of lactobacillus acidophilus in sinbiotic ultrafiltration white cheese containing powdered menthe longifolia l. and spirulina platensis. Iranian Journal of Nutrition Sciences & Food Technology, 9(4), 109-116. (in Persian)
Molan, A.L., Lila, M.A., Mawson, J., & De, S. (2009). In vitro and in vivo evaluation of the prebiotic activity of water-soluble blueberry extracts. World Journal of Microbiology and Biotechnology, 25(7), 1243-1249. doi: https://doi.org/10.1007/s11274-009-0011-9
Mortazavian, A., & Sohrabvandi, S.A. (2006). Textbook of review of probiotic and probiotic products. (1th Ed). Tehran: Ata publisher. (in Persian)
Muir, J.G., Shepherd, S.J., Rosella, O., Rose, R., Barrett, J.S., & Gibson, P.R. (2007). Fructan and free fructose content of common Australian vegetables and fruit. Journal of Agricultural and Food Chemistry, 55(16), 6619-6627.‏ doi: https://doi.org/10.1021/jf070623x
Nguansangiam, S., Angsubhakorn, S., Bhamarapravati, S., & Suksamrarn, A. (2003). Effects of elephant garlic volatile oil (allium ampeloprasum) and T-2 toxin on murine skin. Southeast Asian Journal of Tropical Medicine and Public Health, 34(4), 899-905.
Ocak, E., Javidipour, I., & Tuncturk, Y. (2015). Volatile compounds of van herby cheeses produced with raw and pasteurized milks from different species. Journal of Food Science and Technology, 52(7), 4315-4323.‏ doi: https://doi.org/10.1007/s13197-014-1458-8
Olson, D.W., & Aryana, K.J. (2012). Effect of prebiotics on lactobacillus acidophilus growth and resulting pH changes in skim milk and a model peptone system. Journal of Microbial and Biochemical Technology, 4(5), 121-125.
Ong, L., Henriksson, A., & Shah, N.P. (2007). Chemical analysis and sensory evaluation of cheddar cheese produced with lactobacillus acidophilus, Lb. casei, Lb. paracasei or bifidobacterium sp. International Dairy Journal, 17(8), 937-945. doi: https://doi.org/10.1016/j.idairyj.2007.01.002
Ozcan, O., Ozcan, T., Yilmaz-Ersan, L., Akpinar-Bayizit, A., & Delikanli, B. (2016). The use of prebiotics of plant origin in functional milk products. Food Science and Technology, 4(2), 15-22. doi: https://doi.org/10.13189/fst.2016.040201
Phillips, M., Kailasapathy, K., & Tran, L. (2006). Viability of commercial probiotic cultures (L. acidophilus, Bifidobacterium sp., L. casei, L. paracasei and L. rhamnosus) in cheddar cheese. International Journal of Food Microbiology, 108(2), 276-280. doi: https://doi.org/10.1016/j.ijfoodmicro.2005.12.009
Pourahmad, R., & shaghaghi, M. (2016). Effect of inulin and lactulose on viability of lactobacillus rhamnosus and lactobacillus reuteri and physicochemical characteristics of probiotic set yogurt. Journal of Applied Microbiology in Food Industry, 2(2), 52-60. (in Persian)
Roghani, M., & Aghaie, M. (2007). The effect allium ampeloprasum feeding on serum level of glucose, triglyceride, and total cholesterol of diabetic rats. Koomesh, 8(2), 73-78. (in Persian)
Rosendale, D.I., Maddox, I.S., Miles, M.C., Rodier, M., Skinner, M., & Sutherland, J. (2008). High‐throughput microbial bioassays to screen potential New Zealand functional food ingredients intended to manage the growth of probiotic and pathogenic gut bacteria. International Journal of Food Science & Technology, 43(12), 2257-2267. doi: https://doi.org/10.1111/j.1365-2621.2008.01863.x
Saarela, M., Mogensen, G., Fonden, R., Mättö, J., & Mattila-Sandholm, T. (2000). Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology, 84(3), 197-215. doi: https://doi.org/10.1016/S0168-1656(00)00375-8
Sutherland, J., Miles, M., Hedderley, D., Li, J., Devoy, S., Sutton, K., & Lauren, D. (2009). In vitro effects of food extracts on selected probiotic and pathogenic bacteria. International Journal of Food Sciences and Nutrition, 60(8), 717-727. doi: https://doi.org/10.3109/09637480802165650
Tarakci, Z., Temiz, H., Aykut, U., & Turhan, S. (2011). Influence of wild garlic on color, free fatty acids, and chemical and sensory properties of herby pickled cheese. International Journal of Food Properties, 14(2), 287-299. doi: https://doi.org/10.1080/10942910903176576
Yilmaztekin, M., Özer, B.H., & Atasoy, F. (2004). Survival of lactobacillus acidophilus LA-5 and bifidobacterium bifidum BB-02 in white-brined cheese. International Journal of Food Sciences and Nutrition, 55(1), 53-60. doi: https://doi.org/10.1080/09637480310001642484
Zhang, N., Huang, X., Zeng, Y., Wu, X., & Peng, X. (2013). Study on prebiotic effectiveness of neutral garlic fructan in vitro. Food Science and Human Wellness, 2(3-4), 119-123. doi: https://doi.org/10.1016/j.fshw.2013.07.001
Ziemer, C.J., & Gibson, G.R. (1998). An overview of probiotics, prebiotics and synbiotics in the functional food concept: perspectives and future strategies. International Dairy Journal, 8(5-6), 473-479. doi: https://doi.org/10.1016/S0958-6946(98)00071-5
Zomorodi, SH., Aberun, N., & Khosrowsha Asl, A. (2015). Increase the survival of lactobacillus acidophilus and improved quality properties of senbiotic yogurt using apple and wheat fibers. Food Science and Technology, 12(48), 203-214. (in Persian)
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Volume 7, Issue 4
February 2019
Pages 431-444
  • Receive Date: 21 April 2018
  • Revise Date: 27 October 2018
  • Accept Date: 13 November 2018