پژوهش و نوآوری در علوم و صنایع غذایی
بهینه‌یابی شرایط هیدرولیز پروتئین دانۀ کدو با آنزیم آلکالاز به‌منظور دستیابی به حداکثر فعالیت ضداکسایشی و مهارکنندگی اکسید نیتریک
نوع مقاله : مقاله کامل پژوهشی
نویسندگان
1 دانشجوی کارشناسی ارشد، گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
2 دانشیار، گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
3 استادیار، گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
4 استاد، گروه علوم و صنایع غذایی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان، ایران
چکیده
در این پژوهش، بهینه‌سازی شرایط هیدرولیز پروتئین دانۀ کدو (Cucurbita pepo) به‌منظور دستیابی به حداکثر خصوصیات مهارکنندگی رادیکال 2،2- دی فنیل -1-پیکریل هیدرازیل (DPPH) و مهارکنندگی اکسید نیتریک با استفاده از نرم‌افزار Design Expert و روش سطح پاسخ موردبررسی قرار گرفت. به‌این‌منظور غلظت آنزیم آلکالاز 0/7-3/30 درصد، دمای 32-58 درجۀ سانتی‌گراد و زمان 30-290 دقیقه به‌عنوان سطوح متغیرهای مستقل انتخاب شدند. نتایج نشان داد که شرایط بهینه برای دستیابی به حداکثر خاصیت مهارکنندگی رادیکال DPPH و مهارکنندگی اکسید نیتریک، دمای 44 درجۀ سانتی‌گراد، زمان 260 دقیقه و غلظت آنزیم به سوبسترا 3 درصد و با قابلیت ضداکسایشی و مهارکنندگی اکسید نیتریک برابر با 72/03 و 89/34 درصد بود که تا حدود زیادی مشابه با نتایج پیشنهادشده توسط نرم‌افزار (75/33 و 84/71 درصد) بود. طبق نتایج به‌دست‌آمده پروتئین هیدرولیزشدۀ دانۀ کدو از قابلیت ضداکسایشی و مهارکنندگی اکسید نیتریک مناسبی برخوردار می‌‌باشد.
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دوره 7، شماره 4
اسفند 1397صفحه 445-458
- تاریخ دریافت: 21 اسفند 1396
- تاریخ بازنگری: 01 آبان 1397
- تاریخ پذیرش: 23 آبان 1397
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