Investigating the Effect of Preservatives and Antioxidant on the Oxidative and Microbial Properties of Walnut Butter during the Shelf-life

Document Type : Original Paper

Authors

1 Associate Professor, Department of Food Chemistry, Research Institute of Food Science and Technology, Mashhad, Iran

2 Ph.D. Student, Department of Food Chemistry, Research Institute of Food Science and Technology, Mashhad, Iran

3 M.Sc. Student, Department of Food Science and Technology, Quechan Branch, Islamic Azad University, Quechan, Iran

Abstract

Walnuts contain many nutritional compounds, including omega-3, various vitamins, especially vitamin E and types of minerals. Therefore, the development of foods based on walnut and studies on chemical and microbial stability during storage, is very important. In this research, the effects of various concentrations of BHT (three levels 100, 150 and 200 mg/L) on changes in peroxide value and acid value of walnut butter, as well as the effect of two types of preservatives of benzoic acid and sorbic acid, each in two levels (400 and 800 mg/kg) were separately and mixed, on the total count of microorganisms and the count of mold and yeast of walnut samples during 75 days of storage at ambient temperature is investigated. The results of this study showed that by adding BHT at 200 mg/L, the peroxide value decreased significantly compared to the control sample. After production, acidic value was 0.3% and it rose to 0.4-0.5% after 75 days of storage. Also, benzoic acid was more effective in reducing total bacterial count and counting mold as well as yeast during storage in comparison with sulfuric acid.

Keywords

Abramovič, H., & Abram, V. (2006). Effect of added rosemary extract on oxidative stability of Camelina sativa oil. Acta Agriculturae Slovenica, 87(2), 255-261.
Abu-Jdayil, B., Al-Malah, K., & Asoud, H. (2002). Rheological characterization of milled sesame (tehineh). Food Hydrocolloids, 16(1), 55-61. doi:https://doi.org/10.1016/S0268-005X(01)00040-6
Allen, J., & Hamilton, R. (1994). Rancidity in foods Blackie Academic & Professional Publ. Co. Inc., New York.
AOCS. (1993). Official methods and recommended practices of the American Oil Chemists' Society. 4th ed. Method Aa 6-38. The Society: Champaign, IL.
Bremner, H., Ford, A., Macfarlane, J., Ratcliff, D., & Russell, N. (1976). Meat with high linoleic acid content: Oxidative changes during frozen storage. Journal of Food Science, 41(4), 757-761. doi:https://doi.org/10.1111/j.1365-2621.1976.tb00718_41_4.x
Chien, Y.-h. (2015). Shelf life extension of seed butter made with sesame, sunflower and pumpkin seeds. (Electronic thesis), The Ohio State University. Retrieved from https://etd.ohiolink.edu/ 
Codex Alimentarius Commission. (1993). Proposed draft standard for named vegetable oils. CX 1993/16, issued by the Joint FAO/WHO Food Standards Program, via delle Terme di Caracalla 00100.
Evranuz, E. Ö. (1993). The effects of temperature and moisture content on lipid peroxidation during storage of unblanched salted roasted peanuts: shelf life studies for unblanched salted roasted peanuts. International journal of food science & technology, 28(2), 193-199. doi:https://doi.org/10.1111/j.1365-2621.1993.tb01264.x
Fatemi, H. (2016). Food Chemistry: (pp.480): Enteshar Publication Company. (in Persian)
Gardner, H. (1979). Lipid hydroperoxide reactivity with proteins and amino acids: a review. Journal of Agricultural and Food Chemistry, 27(2), 220-229. doi:https://doi.org/10.1021/jf60222a034
Golzari, M., Rahemi , M., Hassani, D., Vahdati, K., & Mohammadi, N. (2013). Protein content, fat and fatty acids of kernel in some persian walnut (Juglans Regia L.) Cultivars Affected By Kind Of Pollen. Iranian Journal of Food Science And Technology, 10(38), 21-31 (in Persian).
Haghani Haghighi, H., Azar, M., Mazloomi, M., & Komeili, F. (2008). Survey of Formulation, Production and Sensory Evaluation of Pistachio Butter. Food Science and Technology, 5(18), 19-26. (in Persian).
Hasenhuettl, G. L., & Wan, P. J. (1992). Temperature effects on the determination of oxidative stability with the Metrohm Rancimat. Journal of the American Oil Chemists' Society, 69(6), 525-527. doi:https://doi.org/10.1007/BF02636102
Iranian National Standardization Organization. (2001). Pistachio butter - Specification and test methods. (ISIRI Standard No. 5691). Retrieved from http://standard.isiri.gov.ir/StandardFiles/5691.htm (in Persian)
Iranian National Standardization Organization. (2006). Cake –Specification and test methods. (ISIRI Standard No. 2553, 3th Revision). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=11869 (in Persian)
Iranian National Standardization Organization. (2008). Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of yeasts and moulds -Part 1: Colony count technique in products with water activity greater than 0.95. (ISIRI Standard No. 10899.1). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=12277
Iranian National Standardization Organization. (2016a). Microbiology of food and animal feed stuffs-General requirements and guidance for microbiological examinations. (ISIRI Standard No. 9899, 1st Edition). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=43546 (in Persian)
Iranian National Standardization Organization. (2016b). Peanut butter-Specifications and test methods. (ISIRI Standard No. 5690). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=46509 (in Persian)
Jenab, M., Ferrari, P., Slimani, N., Norat, T., Casagrande, C., Overad, K., . . . Boutron-Ruault, M.-C. (2004). Association of nut and seed intake with colorectal cancer risk in the European Prospective Investigation into Cancer and Nutrition. Cancer Epidemiology and Prevention Biomarkers, 13(10), 1595-1603.
Judde, A., Villeneuve, P., Rossignol‐Castera, A., & Le Guillou, A. (2003). Antioxidant effect of soy lecithins on vegetable oil stability and their synergism with tocopherols. Journal of the American Oil Chemists' Society, 80(12), 1209-1215. doi:https://doi.org/10.1007/s11746-003-0844-4
Kaitaranta, J. K. (1992). Control of lipid oxidation in fish oil with various antioxidative compounds. Journal of the American Oil Chemists Society, 69(8), 810-813. doi:https://doi.org/10.1007/BF02635921
Kris-Etherton, P. M., Yu-Poth, S., Sabaté, J., Ratcliffe, H. E., Zhao, G., & Etherton, T. D. (1999). Nuts and their bioactive constituents: effects on serum lipids and other factors that affect disease risk. The American Journal of Clinical Nutrition, 70(3), 504s-511s. doi:https://doi.org/10.1093/ajcn/70.3.504s
Labuza, T. P., & Dugan Jr, L. (1971). Kinetics of lipid oxidation in foods. Critical Reviews in Food Science & Nutrition, 2(3), 355-405. doi:https://doi.org/10.1080/10408397109527127
Law, K. S. (1984). A review on the use of citric acid in the processing of oils and fats. Oleagineux, 39(2), 89-98.
Lazarevic, K., Stojanovic, D., & Rancic, N. (2011). Estimated daily intake of benzoic acid through food additives in adult population of south east Serbia. Central European Journal of Public Health, 19(4), 228. doi:https://doi.org/10.21101/cejph.a3655
Löliger, J. (1990). Headspace gas analysis of volatile hydrocarbons as a tool for the determination of the state of oxidation of foods stored in sealed containers. Journal of the Science of Food and Agriculture, 52(1), 119-128. doi:https://doi.org/10.1002/jsfa.2740520113
Maskan, M., & Karataş, Ş. (1998). Fatty acid oxidation of pistachio nuts stored under various atmospheric conditions and different temperatures. Journal of the Science of Food and Agriculture, 77(3), 334-340. doi:https://doi.org/10.1002/(SICI)1097-0010(199807)77:3
Mureşan, V., Danthine, S., Bolboacă, S. D., Racolţa, E., Muste, S., Socaciu, C., & Blecker, C. (2015). Roasted sunflower kernel paste (tahini) stability: Storage conditions and particle size influence. Journal of the American Oil Chemists' Society, 92(5), 669-683. doi:https://doi.org/10.1007/s11746-015-2622-7
Nahm, H. S., Juliani, H. R., & Simon, J. E. (2012). Effects of selected synthetic and natural antioxidants on the oxidative stability of shea butter (Vitellaria paradoxa subsp. paradoxa). Journal of Medicinally Active Plants, 1(2), 69-75. doi:https://doi.org/10.7275/R5BR8Q4R
Pershern, A. S., Breene, W. M., & Lulai, E. C. (1995). Analysis of factors influencing lipid oxidation in hazelnuts (corylus spp. 1). Journal of Food Processing and Preservation, 19(1), 9-26. doi:https://doi.org/10.1111/j.1745-4549.1995.tb00274.x
Rajaram, S., Haddad, E. H., Mejia, A., & Sabaté, J. (2009). Walnuts and fatty fish influence different serum lipid fractions in normal to mildly hyperlipidemic individuals: a randomized controlled study. The American Journal of Clinical Nutrition, 89(5), 1657S-1663S. doi:https://doi.org/10.3945/ajcn.2009.26736S
Reynhout, G. (1991). The effect of temperature on the induction time of a stabilized oil. Journal of the American Oil Chemists' Society, 68(12), 983-984. doi:https://doi.org/10.1007/BF02657549
Savage, G., Dutta, P., & McNeil, D. (1999). Fatty acid and tocopherol contents and oxidative stability of walnut oils. Journal of the American Oil Chemists' Society, 76(9), 1059-1063. doi:https://doi.org/10.1007/s11746-999-0204-2
Shad, M. A., Zafar, Z. I., Nawaz, H., & Anwar, F. (2012). Effect of sorbic acid and some other food preservatives on human serum cholinesterase activity. African Journal of Biotechnology, 11(51), 11280-11286. doi:http://dx.doi.org/10.5897/AJB11.4037
Shakerardekani, A., Karim, R., Ghazali, H. M., & Chin, N. L. (2015). Oxidative Stability of Pistachio (Pistacia vera L.) Paste and Spreads. Journal of the American Oil Chemists' Society, 92(7), 1015-1021. doi:https://doi.org/10.1007/s11746-015-2668-6
Shantha, N. C., & Decker, E. A. (1994). Rapid, sensitive, iron-based spectrophotometric methods for determination of perorlride values of food lipids. Journal of AOAC International, 77(2), 421-424.
Sharafati Chaleshtori, R., Sharafati Chaleshtori, F., & Rafieian, M. (2011). Biological characterization of Iranian walnut (Juglans regia) leaves. Turkish Journal of Biology, 35(5), 635-639. doi:https://doi/org/10.3906/biy-1005-1
St. Angelo, A. J., & Ory, R. L. (1975). Effects of lipoperoxides on proteins in raw and processed peanuts. Journal of Agricultural and Food Chemistry, 23(2), 141-146. doi:https://doi.org/10.1021/jf60198a028
Williams, G. M., McQueen, C. A., & Tong, C. (1990). Toxicity studies of butylated hydroxyanisole and butylated hydroxytoluene. I. Genetic and cellular effects. Food and Chemical Toxicology, 28(12), 793-798. doi:https://doi.org/10.1016/0278-6915(90)90051-N
CAPTCHA Image
Volume 8, Issue 2
July 2019
Pages 151-164
  • Receive Date: 08 January 2018
  • Revise Date: 20 October 2018
  • Accept Date: 04 November 2018