Study of functional propertiesof protein hydrolysate from White cheek shark (Carcharhinus dussumieri) meat

Document Type : Original Paper

Authors

1 Master of science, Department of Food Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

2 Associate professor, Department of Food Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

3 Assistant professor, Department of Food Science, Sari Agricultural Sciences and Natural Resources University, Sari, Iran

4 Assistant professor, School of Food Science, Washington State University, P.O. Box 646376, Pullman, Washington. USA 99164-6376

Abstract

Protein hydrolysate has been produced from White Cheek Shark meat (Carcharhinus dussumieri) by Alcalase with an activity of 35 Anson Unit/ Kg protein at 55oC. Hydrolysis carried out in four stages each at 10, 20 and 30 minutes. Functional properties such as oil absorption, foaming and emulsion properties, bulk density and viscosity of thehydrolysates examined at three different DHs of 1.91, 2.25 and 2.53%. There were nocorrelation between oil absorption capacity and foaming capacity with DH. Highest values for those properties observed at 2.25% DH (P>0.05).Foam stability decreased during storage at room temperature and the foam expansion totally destroyed after 60 min.Emulsificationcapacity decreased with increasing in DH. Emulsion stability was decreased with increasing in DH and time of storage (P>0.05). Bulk density increased with increasing in DH but the viscosity decreased.However, White Cheek Shark protein hydrolysates showed good functional properties.

Keywords

اویسی‌پور، م.ر. و قمی، م.ر. 1387. بیوتکنولوژی در تولید فراورده‌های دریایی. تنکابن دانشگاه آزاد اسلامی. چاپ اول، ص 98.
معتمدزادگان، ع.، شهیدی، ف.، مرتضوی، ع.، پور آذرنگ، ه.، حمزه، ش.، شهیدی یاساقی، ا.، قربانی حسن سرایی، ا. و خانی پور، ا. 1388. اثر آنزیم پاپائین بر درجه هیدرولیز و طول زنجیره پپتیدی پروتئین‌های میوفیبریلار ماهی کیلکا. مجله علوم کشاورزی و منابع طبیعی، 16(3):1-10.
Carvalho-Silva, L.B.D., Vissotto, F.Z., & Amaya-Farfan, J. 2013. Physico-Chemical properties of milk whey protein agglomerates for use in oral nutritional therapy. Food and Nutrition Sciences, 4:69-78.
Diniz, F.M., & Martin, A.M. 1997b. Optimization of nitrogen recovery in the enzymatic hydrolysis of dogfish (squalus acanthias) protein: Composition of the hydrolysates. International Journal of Food Science and Nutrition, 48:191–200.
Fonkwe, L.G., & Singh, R.K. 1996. Protein recovery from enzymatically deboned turkey residueby enzymic hydrolysis. Journal of  Process Biochemistry, 31:605-616.
Gbogouri, G.A., Linder, M., Fanni, J., & Parmentier, M. 2004. Influence of hydrolysis degree on the functional properties of salmon byproduct hydrolysates. Journal of Food Science, 69:615–622.
Haque, Z.U., & Mozaffar, Z. 1992. Casein hydrolysate. II. Functional properties of peptides. Food Hydrocolloids, 5:559–571.
Kinsella, J.E. 1976. Functional properties of proteins in foods: A survey. Critical Reviews in Food Science and Nutrition, 8:219–280.
Kristinsson, H.G. 1998. Reaction kinetics, biochemical and functional properties of salmon (salmo salar) muscle proteins hydrolyzed by different alkaline proteases. Master thesis, University of Washington, USA.
Kristinsson, H.G., & Rasco, B.A. 2000a. Fish protein hydrolasates: production, biochemical and functional properties. Food Science and Nutritition, 40(1):43–81.
Kristinsson, H.G., & Rasco, B.A. 2000b. Biochemical and functional properties of atlanticsalmon (salmo salar (muscle proteins hydrolyzed with various alkaline proteases. journal of agricultural and food chemistry, 48:657–666.
Klompong, V., Benjakul, S., Kantachote, D., & Shahidi, F. 2007. Antioxidative activity and functional properties of protein hydrolysate of yellow stripe trevally (selaroides leptolepis) as influenced by the degree of hydrolysis and enzyme type. Food Chemistry,102(4):1317-1327.
Liaset, B., Nortvedt, R., Lied, E., & Espe, M. 2002. Studies on the nitrogen recovery in enzymic hydrolysis of Atlantic salmon (salmo salar, L.) frames by Protamex protease. Process Biochemistry, 37:1263–1269.
Liceaga-Gesualdo, A.M, & Li-Chan, E.C.Y. 1999. Functional properties of fish proteinhydrolysate from herring (clupea harengus). Journal of food science, 64:1000-1004.
Lone, D.A., Wani, N.A., Wani, I.A., & Masoodi, F.A. 2015. Physico-chemical and functional properties of rainbow trout fish protein isolate. International Food Research Journal, 22(3):1112-1116.
Mahmoud, M.I. 1994. Physicochemical and functional properties of protein hydrolysates in nutritional products. Food Technology, 58(10):89-95.
Onadenalore, A., & Shahidi, F. 1996. Protein dispersions and hydrolysates from shark (isurus oxyrinchus).Journal of Aquatic product Technology, 5(4):43-59.
Ovissipour, M., Abedian, A., Motamedzadegan, A., Rasco, B., Safari, R., & Shahiri, H.  2009. The effect of enzymatic hydrolysis time and temperature on the properties of protein hydrolysatesfromPersian sturgeon (acipenser persicus) viscera. Journal of Food Chemistry, 115:238-242.
Phillips, L.G., Whitehead, D.M., & Kinsella, J.E. 1994. Protein stabilized foams. In L. G. Phillips, D. M. Whitehead, and J. E. Kinsella (Eds.), Structure-function of food proteins (pp. 131–152). New York, USA: Academic Press.
Quaglia, G.B., & Orban, E. 1987. Enzymicsolubilisation of proteins of sardine (sardina pilchardus) by commercial proteases. Journal of the Science of Food and Agriculture, 38:263–269.
Rahali, V., Chobert, J.M., Haertle, T., & Gueguen, J. 2000. Emulsification of chemical and enzymatic hydrolysates of beta-lactoglobulin: characterization of the peptides adsorbed at the interface. Nahrung, 44:89–95.
Regenstein, J.M., & Regenstein, C.E. 1984. Diffusion and viscosity. In: food protein chemistry. an introduction for food scientists. Orlando, FL. Academic Press, Inc, 224-228.
Sathivel, S., Yin, H., Bechtel, P.J., & King, M.J. 2009. Physical and nutritional properties of catfish roe spray dried protein powder and its application in an emulsion system. Journal of Food Engineering, 95:76–81.
Shahidi, F., Han, X.Q., & Synowiecki, J. 1995. Production and characteristic of protein hydrolysates from capelin (Mallotus villosus). Food chemistry, 53:285- 293.
Slizyte, R., Mozuraitytė, R., Martínez-Alvarez, O., Falch, E., Fouchereau-Peron, M., & Rustad, T. 2009. Functional, bioactive and antioxidative properties of hydrolysates obtained from cod (gadus morhua) Backbones. Process Biochemistry, 44:668-677.
Souissi, N., Bougatef, A., Triki-Ellouz, Y., & Nasri, M. 2007. Biochemical and functional properties of sardinella(sardinella aurita)by-product hydrolysates. Food Technology Biotechnology, 45(2):187–194.
Surowka, K., & Fik, M. 1992. Studies on the recovery of proteinaceous substances from chicken heads. i. An application of neutrase to the production of protein hydrolysate. International Journal of Food Science andTechnology, 27:9–20.
Surowka, K., & Fik, M. 1994. Studies on the recovery of proteinaceous substances from chicken heads: II- Application of pepsin to the production of protein hydrolysate. Journal of the Science of Food and Agriculture, 65:289–296.
Wang, J.C., & Kinsella, J.E. 1976. Functional properties of novel proteins: Alfalfa leaf protein. Journal of Food Science, 41:286–292 .
Wasswa, J., Tang, J., Gu, X., & Yuan., X. 2007. Influence of the extent of enzymatic hydrolysis on the functional properties of protein hydrolysate from grass carp (ctenopharyngodon idella) skin. Food Chemistry, 104:1698–1704.
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Volume 5, Issue 1
June 2016
Pages 27-38
  • Receive Date: 05 September 2014
  • Revise Date: 07 November 2015
  • Accept Date: 15 November 2015