Physicochemical Properties, Phenolic Contents and Antioxidant Activity of Vietnamese Honey

Pham, Tri Nhut; Le, Hoang Thien; Nguyen, Quang Vinh; Tran, Quoc Toan; Tran, Minh Phat

doi:10.22101/JRIFST.2021.295084.1258

Physicochemical Properties, Phenolic Contents and Antioxidant Activity of Vietnamese Honey

Document Type : Original Paper

Authors

¹ MSc. Graduate, Institute of Applied Technology and Sustainable Development, Nguyen Tat Thanh University, Ho Chi Minh City, Vietnam

² MSc. Graduate, Faculty of Process and Systems Engineering, Otto-von-Guericke-Universität Magdeburg, Germany

³ Associate Professor, Institute of Biotechnology and Environment, Tay Nguyen University, Dak Lak Province, Vietnam

⁴ PhD. Graduate, Institute of Natural Products Chemistry, Vietnam Academy of Science and Technology (VAST), Hanoi City, Vietnam

⁵ BSc. Graduate, Tin Phat Honey Joint Stock Company, Ben Tre Province, Vietnam

10.22101/JRIFST.2021.295084.1258

Abstract

Vietnam is located in the tropical monsoon climate area (Southeast Asia), along with the biodiversity of plant species, which has created many types of monofloral and polyfloral honey. However, there has not been a study that fully reports on the physicochemical composition, antioxidant activity and content of phytochemical compounds of honey in this country. Therefore, this study aimed to determine Vietnam's physicochemical properties, antioxidant activity, and phytochemical content of longan flower honey and natural polyfloral honey (from Micrapis honeycomb). The reference analytical methods were modified based on previous honey studies. Following proximate indicators were found: The moisture content varied from 21.9 to 30.5%, the ash content varied from 0.37 to 0.48%, the protein content varied from 0.25 to 0.41%, and the 5-(hydroxymethyl)-2-furaldehyde (HMF) varied from 33.4 to 75.4%. The total phenolic and flavonoid concentrations ranged from 0.890 to 1.110 mgGAE/g and 0.049 to 0.089 mgQE/g. Besides, the antioxidant activity of longan flower honey was recorded as the best, with the IC₅₀ value of 68.49 mg/mL. In addition, Water content, free acidity, sugar content (glucose, fructose, and sucrose), and HMF concentration were all tested and presented.

Keywords

References

Abdulkhaliq, A., & Swaileh, K. M. (2017). Physico-chemical properties of multi-floral honey from the West Bank, Palestine. International Journal of Food Properties, 20(2), 447-454. doi:https://doi.org/10.1080/10942912.2016.1166128

Ajlouni, S., & Sujirapinyokul, P. (2010). Hydroxymethylfurfuraldehyde and amylase contents in Australian honey. Food Chemistry, 119(3), 1000-1005. doi:https://doi.org/10.1016/j.foodchem.2009.07.057

Al-Farsi, M., Al-Amri, A., Al-Hadhrami, A., & Al-Belushi, S. (2018). Color, flavonoids, phenolics and antioxidants of Omani honey. Heliyon, 4(10), e00874-e00874. doi:https://doi.org/10.1016/j.heliyon.2018.e00874

Al, M. L., Daniel, D., Moise, A., Bobis, O., Laslo, L., & Bogdanov, S. (2009). Physico-chemical and bioactive properties of different floral origin honeys from Romania. Food Chemistry, 112(4), 863-867. doi:https://doi.org/10.1016/j.foodchem.2008.06.055

Alvarez-Suarez, J. M., Tulipani, S., Díaz, D., Estevez, Y., Romandini, S., Giampieri, F., . . . Battino, M. (2010). Antioxidant and antimicrobial capacity of several monofloral Cuban honeys and their correlation with color, polyphenol content and other chemical compounds. Food Chem Toxicol, 48(8-9), 2490-2499. doi:https://doi.org/10.1016/j.fct.2010.06.021

Alzahrani, H. A., Boukraa, L., Bellik, Y., Abdellah, F., Bakhotmah, B. A., Kolayli, S., & Sahin, H. (2012). Evaluation of the antioxidant activity of three varieties of honey from different botanical and geographical origins. Global journal of health science, 4(6), 191-196. doi:https://doi.org/10.5539/gjhs.v4n6p191

Anklam, E. (1998). A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistry, 63(4), 549-562. doi:https://doi.org/10.1016/S0308-8146(98)00057-0

AOAC. (2000). Official Methods of Analysis: The Association of Official Analytical Chemists, Gaithersburg, MD, USA. Methods 925.10, 65.17, 974.24, 992.16. .

Bertoncelj, J., Doberšek, U., Jamnik, M., & Golob, T. (2007). Evaluation of the phenolic content, antioxidant activity and colour of Slovenian honey. Food Chemistry, 105(2), 822-828. doi:https://doi.org/10.1016/j.foodchem.2007.01.060

Chakir, A., Romane, A., Marcazzan, G. L., & Ferrazzi, P. (2016). Physicochemical properties of some honeys produced from different plants in Morocco. Arabian Journal of Chemistry, 9, S946-S954. doi:https://doi.org/10.1016/j.arabjc.2011.10.013

Cheung, Y., Meenu, M., Yu, X., & Xu, B. (2019). Phenolic acids and flavonoids profiles of commercial honey from different floral sources and geographic sources. International Journal of Food Properties, 22(1), 290-308. doi:https://doi.org/10.1080/10942912.2019.1579835

Chirife, J., Zamora, M. C., & Motto, A. (2006). The correlation between water activity and % moisture in honey: Fundamental aspects and application to Argentine honeys. Journal of Food Engineering, 72(3), 287-292. doi:https://doi.org/10.1016/j.jfoodeng.2004.12.009

Codex Alimentarius. (2001). Revised codex standard for honey. Codex stan, 12, 1982.

Combarros-Fuertes, P., Estevinho, L. M., Dias, L. G., Castro, J. M., Tomás-Barberán, F. A., Tornadijo, M. E., & Fresno-Baro, J. M. (2019). Bioactive Components and Antioxidant and Antibacterial Activities of Different Varieties of Honey: A Screening Prior to Clinical Application. J Agric Food Chem, 67(2), 688-698. doi:https://doi.org/10.1021/acs.jafc.8b05436

Conti, M. E., Stripeikis, J., Campanella, L., Cucina, D., & Tudino, M. B. (2007). Characterization of Italian honeys (Marche Region) on the basis of their mineral content and some typical quality parameters. Chemistry Central journal, 1, 14-14. doi:https://doi.org/10.1186/1752-153X-1-14

Council, E. (2002). Council Directive 2001/110/EC of 20 December 2001 relating to honey. Official Journal of the European Communities L, 10, 47-52.

Da Costa Leite, J. M., Trugo, L. C., Costa, L. S. M., Quinteiro, L. M. C., Barth, O. M., Dutra, V. M. L., & De Maria, C. A. B. (2000). Determination of oligosaccharides in Brazilian honeys of different botanical origin. Food Chemistry, 70(1), 93-98. doi:https://doi.org/10.1016/S0956-7135(99)00115-2

da Silva, P. M., Gauche, C., Gonzaga, L. V., Costa, A. C. O., & Fett, R. (2016). Honey: Chemical composition, stability and authenticity. Food Chemistry, 196, 309-323. doi:https://doi.org/10.1016/j.foodchem.2015.09.051

DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., & Smith, F. (1956). Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry, 28(3), 350-356. doi:https://doi.org/10.1021/ac60111a017

Finola, M. S., Lasagno, M. C., & Marioli, J. M. (2007). Microbiological and chemical characterization of honeys from central Argentina. Food Chemistry, 100(4), 1649-1653. doi:https://doi.org/10.1016/j.foodchem.2005.12.046

Hailu, D., & Belay, A. (2020). Melissopalynology and antioxidant properties used to differentiate Schefflera abyssinica and polyfloral honey. PLOS ONE, 15(10), e0240868. doi:https://doi.org/10.1371/journal.pone.0240868

Halliwell, B. (2007). Cellular responses to oxidative stress: adaptation, damage, repair, senescence and death (Vol. 4^rd): Oxford University Press, New York.

Hamdy, A. A., Ismail, H. M., Al-Ahwal Ael, M., & Gomaa, N. F. (2009). Determination of flavonoid and phenolic Acid contents of clover, cotton and citrus floral honeys. J Egypt Public Health Assoc, 84(3-4), 245-259.

Hatano, T., Kagawa, H., Yasuhara, T., & Okuda, T. (1988). Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem Pharm Bull (Tokyo), 36(6), 2090-2097. doi:https://doi.org/10.1248/cpb.36.2090

Idris, Y. M. A., Mariod, A. A., & Hamad, S. I. (2011). Physicochemical Properties, Phenolic Contents and Antioxidant Activity of Sudanese Honey. International Journal of Food Properties, 14(2), 450-458. doi:https://doi.org/10.1080/10942910903243673

Isla, M. I., Craig, A., Craig, A., Ordoñez, R., Zampini, C., Sayago, J., . . . Maldonado, L. (2011). Physico chemical and bioactive properties of honeys from Northwestern Argentina. Lebensmittel-Wissenschaft + [i.e. und] Technologie, 44(9), 1922-1930. doi:https://doi.org/10.1016/j.lwt.2011.04.003

Islam, A., Khalil, I., Islam, N., Moniruzzaman, M., Mottalib, A., Sulaiman, S. A., & Gan, S. H. (2012). Physicochemical and antioxidant properties of Bangladeshi honeys stored for more than one year. BMC Complementary and Alternative Medicine, 12(1), 177. doi:https://doi.org/10.1186/1472-6882-12-177

Ita, B. N. (2011). Antioxidant activity of honey samples from the southern rainforest and northern savannah ecosystems in nigeria. International Journal of Pharmaceutical Sciences and Research, 2(8), 2115-2120.

Khalil, M. I., Sulaiman, S. A., Alam, N., Ramli, N. S., Mohamed, M., Ba‰ e, S., & Hua, G. S. (2012). Content and antioxidant properties of processed tualang honey (Agromas®) collected from different regions in malaysia. International Journal of Pharmacy and Pharmaceutical Sciences, 4(3), 214-219.

Khalil, M. I., Sulaiman, S. A., & Gan, S. H. (2010). High 5-hydroxymethylfurfural concentrations are found in Malaysian honey samples stored for more than one year. Food and Chemical Toxicology, 48(8), 2388-2392. doi:https://doi.org/10.1016/j.fct.2010.05.076

Kortesniemi, M., Rosenvald, S., Laaksonen, O., Vanag, A., Ollikka, T., Vene, K., & Yang, B. (2018). Sensory and chemical profiles of Finnish honeys of different botanical origins and consumer preferences. Food Chemistry, 246, 351-359. doi:https://doi.org/10.1016/j.foodchem.2017.10.069

Le, X. T., Huynh, M. T., Pham, T. N., Than, V. T., Toan, T. Q., Bach, L. G., & Trung, N. Q. (2019). Optimization of Total Anthocyanin Content, Stability and Antioxidant Evaluation of the Anthocyanin Extract from Vietnamese Carissa Carandas L. Fruits. Processes, 7(7), 468-. doi:https://doi.org/10.3390/pr7070468

Liu, H., Qiu, N., Ding, H., & Yao, R. (2008). Polyphenols contents and antioxidant capacity of 68 Chinese herbals suitable for medical or food uses. Food Research International, 41(4), 363-370. doi:https://doi.org/10.1016/j.foodres.2007.12.012

Machado De-Melo, A. A., Almeida-Muradian, L. B. d., Sancho, M. T., & Pascual-Maté, A. (2018). Composition and properties of Apis mellifera honey: A review. Journal of Apicultural Research, 57(1), 5-37. doi:https://doi.org/10.1080/00218839.2017.1338444

Machu, L., Misurcova, L., Ambrozova, J. V., Orsavova, J., Mlcek, J., Sochor, J., & Jurikova, T. (2015). Phenolic content and antioxidant capacity in algal food products. Molecules, 20(1), 1118-1133. doi:https://doi.org/10.3390/molecules20011118

Marinova, D., Ribarova, F., & Atanassova, M. (2005). Total phenolics and total flavonoids in bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy,, 40(3), 255-260.

Miller, G. L. (1959). Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Analytical Chemistry, 31(3), 426-428. doi:https://doi.org/10.1021/ac60147a030

Moniruzzaman, M., Khalil, M. I., Sulaiman, S. A., & Gan, S. H. (2013). Physicochemical and antioxidant properties of Malaysian honeys produced by Apis cerana, Apis dorsata and Apis mellifera. BMC Complementary and Alternative Medicine, 13(1), 43. doi:https://doi.org/10.1186/1472-6882-13-43

Moniruzzaman, M., Sulaiman, S. A., Khalil, M. I., & Gan, S. H. (2013). Evaluation of physicochemical and antioxidant properties of sourwood and other Malaysian honeys: a comparison with manuka honey. Chemistry Central journal, 7, 138-138. doi:https://doi.org/10.1186/1752-153X-7-138

Ouchemoukh, S., Louaileche, H., & Schweitzer, P. (2007). Physicochemical characteristics and pollen spectrum of some Algerian honeys. Food Control, 18(1), 52-58. doi:https://doi.org/10.1016/j.foodcont.2005.08.007

Pham, T. N., Nguyen, V. T., Toan, T. Q., Cang, M. H., Bach, L. G., & Van Muoi, N. (2020). Effects of Various Processing Parameters on Polyphenols, Flavonoids, and Antioxidant Activities of Codonopsis javanica Root Extract. Natural Product Communications, 15(9), 1934578X20953276. doi:https://doi.org/10.1177/1934578x20953276

Sakač, M. B., Jovanov, P. T., Marić, A. Z., Pezo, L. L., Kevrešan, Ž. S., Novaković, A. R., & Nedeljković, N. M. (2019). Physicochemical properties and mineral content of honey samples from Vojvodina (Republic of Serbia). Food Chemistry, 276, 15-21. doi:https://doi.org/10.1016/j.foodchem.2018.09.149

Saxena, S., Gautam, S., & Sharma, A. (2010). Physical, biochemical and antioxidant properties of some Indian honeys. Food Chemistry, 118(2), 391-397. doi:https://doi.org/10.1016/j.foodchem.2009.05.001

Singh, N., & Bath, P. K. (1997). Quality evaluation of different types of Indian honey. Food Chemistry, 58(1), 129-133. doi:https://doi.org/10.1016/S0308-8146(96)00231-2

Socha, R., Juszczak, L., Pietrzyk, S., & Fortuna, T. (2009). Antioxidant activity and phenolic composition of herbhoneys. Food Chemistry, 113(2), 568-574. doi:https://doi.org/10.1016/j.foodchem.2008.08.029

Tewari, J., & Irudayaraj, J. (2004). Quantification of Saccharides in Multiple Floral Honeys Using Fourier Transform Infrared Microattenuated Total Reflectance Spectroscopy. J Agric Food Chem, 52(11), 3237-3243. doi:https://doi.org/10.1021/jf035176+

Toan, T. Q., Phong, T. D., Tien, D. D., Linh, N. M., Mai Anh, N. T., Hong Minh, P. T., . . . Tung, N. Q. (2021). Optimization of Microwave-Assisted Extraction of Phlorotannin From Sargassum swartzii (Turn.) C. Ag. With Ethanol/Water. Natural Product Communications, 16(2), 1934578X21996184. doi:https://doi.org/10.1177/1934578x21996184

Wanjai, C., Sringarm, K., Santasup, C., Pak-Uthai, S., & Chantawannakul, P. (2012). Physicochemical and microbiological properties of longan, bitter bush, sunflower and litchi honeys produced by Apis mellifera in Northern Thailand. Journal of Apicultural Research, 51(1), 36-44. doi:https://doi.org/10.3896/IBRA.1.51.1.05

White, J. W., Jr., & Maher, J. (1953). Transglucosidation by honey invertase. Arch Biochem Biophys, 42(2), 360-367. doi:https://doi.org/10.1016/0003-9861(53)90365-8

Zhishen, J., Mengcheng, T., & Jianming, W. (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4), 555-559. doi:https://doi.org/10.1016/S0308-8146(98)00102-2