نوع مقاله : مقاله کامل پژوهشی

نویسندگان

1 گروه میکروبیولوژی، واحد آیت‌الله آملی، دانشگاه آزاد اسلامی، آمل، ایران

2 گروه میکروبیولوژی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران

3 گروه باکتری‌شناسی، دانشکده دامپزشکی، دانشگاه تخصصی فناوری‌های نوین، آمل، ایران

4 گروه میکروب‌شناسی، دانشکده علوم پایه، واحد اهر، دانشگاه آزاد اسلامی، اهر، ایران

چکیده

آرکوباکتر بوتزلری رایج‌ترین جنس از خانوادۀ کمپیلوباکتریاسه است که به‌عنوان پاتوژن زئونوز و نوظهور شناخته شده است. هدف از این مطالعه جداسازی، شناسایی و تعیین حساسیت ضدمیکروبی سویه‌های آرکوباکتر بوتزلری نسبت به آنتی‌بیوتیک‌هایی است که در درمان بیماری‌های عفونی در انسان و حیوانات استفاده می‌شود. ازاین‌‌رو، 297 نمونه از لاشۀ مرغ در کشتارگاه‌های شهرستان تنکابن جمع‌آوری شد. کُلنی‌های مشکوک با استفاده از تست بیوشیمیایی جداسازی و شناسایی شدند و از تکنیک واکنش زنجیره‌ای پلی‌مراز برای تکثیر ژن 16SrRNA و تأیید نتایج فتوتیپی استفاده شد. الگوی مقاومت آنتی‌بیوتیکی آرکوباکتر بوتزلری نسبت به 16 آنتی‌بیوتیک به روش دیسک دیفیوژن و تعیین حداقل غلظت مهارکنندۀ رشد سویه‌ها نسبت به تتراسایکلین، اریترومایسین و جنتامایسین به روش رقت‌سازی لوله‌ای تعیین گردید. از 36 سویۀ جداسازی و شناسایی‌شده، تمامی جدایه‌ها نسبت به پنی‌سیلین 100 درصد، آمپی‌سیلین 100 درصد و اگزاسیلین 100 درصد مقاومت نشان دادند و همچنین میزان مقاومت به تری‌متوپریم/سولفامتوکسازول 94/4 درصد، سیپروفلوکساسین 94/4 درصد، نالیدیکسیک اسید 91/7 درصد، آزیترومایسین 91/7 درصد و آموکسی‌سیلین 80/6 درصد ارزیابی گردید. از 36 جدایۀ آزمایش‌شده، تمامی ایزوله‌ها نسبت به جنتامایسین 100 درصد حساسیت نشان داده‌اند. 72 درصد سویه‌ها دارای حداقل غلظت مهارکنندگی≥128 (میکروژول بر میلی‌‌لیتر) و حداقل غلظت کشندگی≥256 (میکروژول بر میلی‌‌لیتر) نسبت به آنتی‌بیوتیک تتراسایکلین بودند. همچنین تعداد 10 سویۀ مقاومت دارویی چندگانه (27/77 درصد) و تعداد 24 سویۀ مقاومت دارویی گسترده (66/66 درصد) بوده است. یافته‌های تحقیق حاضر، وجود آرکوباکتر بوتزلری را در لاشه‌های مرغ و شیوع بالای مقاومت ضدمیکروبی نسبت به آنتی‌بیوتیک‌های مختلف را در این ناحیه نشان داد.

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