Introduction: Flavonoids are naturally occurring plant molecules. Fisetin is a bioactive polyphenolic flavanol molecule found in fruits and vegetables with potential pharmacological activities. This study was designed to validate a simple method for the estimation of fisetin in the strawberry extract by spectrophotometry.
Objective: Validation of UV-Visible spectrophotometric method for estimation of fisetin.
Methodology: Isolation of fisetin done by the powder of Fragaria ananassa with chloroform. The wet granulation method was formulated for the tablet by incorporating pure fisetin as a biomarker and isolated separately. Spectrophotometric detection was carried out at an absorption maximum of 362 nm using methanol as solvent. The method was validated for linearity, accuracy, and precision.
The drug, fisetin obeys the Beer-Lambert’s law in the concentration range of 1-8 µg/mL and was found to be linear with a correlation coefficient of 0.9927, and the regression equation was found to be Y= 0.1015x-0.043. Found the percentage relative standard deviation among all the responses was less than 2% indicating the precision of the method. The percentage recovery of fisetin was found to be in the range of 96.61-101.63 %.
Conclusion and highlights
The present study was statistically confirmed. Therefore, the proposed approach can be used to measure the active marker compound in the crude drug accurately. The study allows the optimization of a precise method for the determination of the fisetin in the fruit of fragaria ananassa, which can be used to support the quality assessment of this herbal material.