نوع مقاله: مقاله کامل پژوهشی

نویسندگان

1 دانشجوی دوره دکتری تخصصی، گروه شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

2 دانشیار، گروه شیلات، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

3 استاد، گروه اصلاح نژاد گیاهی و بیوتکنولوژی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، ساری، ایران

چکیده

کمّیت و کیفیت چربی استخراج‌شده از ریزجلبک‌ها شدیداً متأثر از روش انتخابی برای تخریب و شکستن سلول‌ها و نوع حلال می‌باشد. در این پژوهش با مقایسۀ چندین ماده و روش متفاوت، شامل آنزیم (سلولاز و فلورزایم)، التراسوند و هموژنایزر با سطوح مختلف و حلال‌های آب، اتانول و متانول مؤثرترین شیوۀ تخریب سلول‌های ریزجلبکی Dunaliella Salina و بهترین حلال به‌منظور استخراج اسیدهای چرب موردبررسی قرار گرفت. براساس نتایج آنزیمی میزان روغن استخراجی با سطح سلولاز 3 درصد و فلورزایم 1/5 درصد برابر 2/04 گرم بر لیتر برحسب وزن خشک جلبک، التراسوند میزان روغن استخراجی 1/61 گرم بر لیتر در مدت زمان 3 دقیقه و ترکیب روش‌های آنزیمی و هموژنایزر 2/26 گرم بر لیتر ثبت گردید. ازطرفی میزان اسیدهای چرب C18:1، C16:0 و C18:2 استخراجی در بین 3 حلال مختلف اتانول به‌ترتیب به میزان 8/22، 1/08 و 5/18 درصد ثبت گردید. نتایج به‌دست‌آمده از این پژوهش، ترکیب روش‌های آنزیمی و هموژنایزر برای تخریب دیوارۀ سلولی و استخراج اسیدهای چرب با حلال اتانول را به‌‌عنوان راهکاری مناسب و مؤثر پیشنهاد می‌نماید.

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