Document Type : Original Paper

Authors

• Sokoofeh Heydari ¹
• Mostafa Shahidi Noghabi ²
• Mohammad Reza Abdollahi Moghaddam ²

¹ Department of Chemical Engineering, College of Engineering, Shahrood Branch, Islamic Azad University, Shahrood, Iran

² Department of Food Chemistry, Research Institute of Food Science and Technology, Mashhad, Iran

Abstract

Nowadays, due to the environmental problems caused by packaging based on synthetic polymers, the use of producing edible films based on biopolymers like polysaccharides, proteins, and fats has been interested. According to all the useful features of psyllium seed hydrocolloid (PH), the aim of this study was to evaluate the possibility of antimicrobial biodegradable edible film production using PH, glycerol (as plasticizer), nisin and EDTA (as antimicrobial). The films were prepared with different levels of nisin (0, 5000 and 10000 IU/g) and EDTA (0, 15 and 30 wt%). The diameter of the inhibition zones against pathogenic bacteria on medium surface were considered as a measure of film antimicrobial activity. The results showed that films containing nisin and EDTA significantly prevent the activity of Staphylococcus aureus (as a Gram-positive bacteria) and Salmonella typhimurium (as a Gram-negative bacteria). With increasing concentration of nisin and EDTA the inhibitory power of film against bacteria increased. Maximum inhibition was observed at 3416.03 IU/g concentration of nisin and highest concentration of EDTA (30%). At the optimal point, the inhibited zone for Staphylococcus aureus and Salmonella typhimurium was 2.02±0.2 and 2.2±0.3cm respectively. Physical and mechanical properties of the optimal film were measured and reported. According to results of this study, These novel biodegradable antimicrobial films could be suggest as meat product packaging.

Keywords

• EDTA
• Nisin
• Psyllium seed Hydrocolloid
• Edible film

Main Subjects

• Food Packaging