Evaluation of bene hull extraction antioxidant activity by superheated water method

Document Type : Original Paper

Authors

1 MSc. Student, Department Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad

2 Professor, Department of Food Science and Technology, Agriculture College, Ferdowsi University of Mashhad

3 Assistant Professor, Department of Food Processing, Research Institute of Food Science and Technology

4 Assistant Professor, Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad

5 Assistant Professor, Department of Food Science and Technology, College of Agriculture, University of Tabriz

Abstract

In the present research, the power of antioxidant activity in Bene Hull subspecies (Mutica) extract was discussed using subcritical water technique, at 110,155 and 200°C temperatures with 45 minutes of constant time and the ratio of  for Bene hull to water. The dependent variables of this study were measured based on Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) and total amount of phenolic compounds (Mg of Gallic Acid/ 100 G of material). The study revealed that at 200°C the Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) were at the maximum; meanwhile total Phenolic content was highest at 155°C. Under this circumstance, the amount of polyphenolic contents, reduction power according to EC50 and DPPH free radical scavenging activity according to EC50 were predicted to be 2096.41 mg Gallic acid / 100 g Bene hull, 0.213 mg /ml and 0.685 mg/ ml, respectively. The results proved that subcritical water extraction method was suitable to obtain biological materials out of Bene hull. Intense extraction yield, abundant solvent, cheap and non-environmental issues are its benefits.
In the present research, the power of antioxidant activity in Bene Hull subspecies (Mutica) extract was discussed using subcritical water technique, at 110,155 and 200°C temperatures with 45 minutes of constant time and the ratio of

 for Bene hull to water. The dependent variables of this study were measured based on Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) and total amount of phenolic compounds (Mg of Gallic Acid/ 100 G of material). The study revealed that at 200°C the Melanoidic impact extent, the power of resuscitating ferrous (Mg/Ml) the extent of controlling DPPH free radicals (Mg/ Ml) were at the maximum; meanwhile total Phenolic content was highest at 155°C. Under this circumstance, the amount of polyphenolic contents, reduction power according to EC50 and DPPH free radical scavenging activity according to EC50 were predicted to be 2096.41 mg Gallic acid / 100 g Bene hull, 0.213 mg /ml and 0.685 mg/ ml, respectively. The results proved that subcritical water extraction method was suitable to obtain biological materials out of Bene hull. Intense extraction yield, abundant solvent, cheap and non-environmental issues are its benefits

Keywords

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Volume 1, Issue 2
June 2012
Pages 75-86
  • Receive Date: 29 May 2011
  • Revise Date: 25 September 2011
  • Accept Date: 02 October 2011