Optimization of low fat ice cream formulation using microbial transglutaminase and protein based fat replacers

Document Type : Original Paper

Authors

1 MSc. Graduated Student, Department of Food Science and Technology, Agricultural Sciences and Natural Resource University of Gorgan, Iran

2 Associate Professor, Department of Food Science and Technology, Agricultural Sciences and Natural Resource University of Gorgan , Iran

3 Associate Professor, Department of Food Science and Technology, Agricultural Sciences and Natural Resource University of Sari , Iran

4 PhD. Student, Department of Food Microbiology, Malayer University

Abstract

In this study whey protein concentrate (WPC) at levels (0.1and 4%), soy protein isolate (SPI) at levels (0.1and 2%) and microbial transglutaminase (MTG) at levels (0.1and 2%) were used in low fat ice cream formulation (4% fat) and their effects were evaluated on thephysicochemical and overall acceptance and their optimum amount was determined using response surface methodology. The amount of total solids in all samples was the same (34%). According to the results formulations containing WPC had higher viscosity and acidity but lower overrun, melting resistant and overall acceptance compared to the control sample (P<0.01). SPI resulted in greater viscosity and melting resistance but lower overrun and overall acceptance (P<0.01). MTG resulted in greater melting resistance, overrun and viscosity of the ice cream samples in comparison to control sample (P<0.01). But had no significant effects on overall acceptance and acidity (P>0.05). The result of optimization showed that the best levels of SPI, WPC and MTG were 0.06, 1.37 and 1.19 % respectively. Also in optimum levels, the amount of viscosity, melting resistance, overrun, acidity and overall acceptance were obtained 1700 cp, 52%, 49/54%, 28/5 dornik and 4.25, respectively.

Keywords

پروانه، و. 1385. کنترل کیفی و آزمایش‌های شیمیایی مواد غذایی. چاپ سوم. موسسه چاپ و انتشارات دانشگاه تهران. صفحه 332.
چگنی، ب. و مشکوه، آ. 1385. تاریخچه پیدایش بستنی در دانش و تکنولوژی بستنی. آییژ، تهران، ایران، 2.
خشایار، پ. 1385. غنی‌سازی بستنی کم‌چرب با سویا. شانزدهمین کنگره ملی صنایع غذایی ایران. دانشگاه علوم کشاورزی و منابع طبیعی گرگان.
رضوی، س. م.، حبیبی نجفی، م. ب. و نایب زاده، ک. 1379. تاثیر جایگزین‌های لبنی و نوع پایدار‌کننده بر خصوصیات شیمیایی و فیزیکی بستنی سویا. مجله علوم کشاورزی ایران. 32(3): 624-615.
همایونی‌راد، ع.، احسانی، م.ر.، ابراهیم‌زاده موسوی، م.ع.، ولی‌زاده، م.، و امام جمعه، ز. 1383. بهبود کیفیت بستنی کم‌چرب به کمک هیدرولیز نسبی پروتئین‌های مخلوط بستنی با کیموزین (II). مجله علوم کشاورزی ایران، 36 (6): 1509- 1515.
Akalin, A.S. & Erisir, D. 2008. Effects of inulin and oligofructose on the rheological characteristics and probiotic culture survival in low fat probiotic ice cream. Food Science and Technology, 731: 184–188.
Akalinm, A. S., Karagozlu, C. & Unal, G. 2008. Rheological properties of reduced-fat and low-fat ice cream containing whey protein isolate and inulin. European Food Research and Technology, 227: 889-895.
Akesowan, A. 2009. Influence of Soy protein isolate on physical and sensory properties of ice cream. Agricultural Science, 42: 1-6.
Ando, H., Adachi, M., Umeda, K., Matsuura, A., Nonaka, M., Uchio, Tanaka, H. & Motoki, M. 1989. Purification and characteristics of a novel transglutaminase derived from microorganisms. Agricultural and Biological Chemistry, 53: 2613–17.
Arbuckle, W. S. 197. Ice cream. (3rd Ed.). Connecticut: Avi Publisher Company.
Arrese, E.L., Sorgentini, D.A., Wagner, J.R. & M.C, Anon. 1991. Electrophoretic, solubility and functional properties of commercial soy protein isolates. Journal of Agricultural and Food Chemistry, 39 (6): 1029–1032.
Athetron, H. V. & Newlander, J.A. 1997. Chemistry and testing of dairy products, 4th Ed., AVI Publ., Westport, CT.
Berger, K. G.1990. Ice cream in food emulsions, 2nd Ed.. In: Lars-son K, Friberg SE (eds) Marcel Dekker, Inc., New York, 367.
Bringe, N.A. & Clark, D.R .1993. Simplesse ® formulation and properties of microparticulated whey protein. In: M Yalpani (Ed), Science for the food industry of the 21st Century, ATL Press, Mt. Prospect, IL: 51–68.
Dervisoglu, M., Yazici, F. & Aydemir, O. 2005. The effect of soy protein concentrate addition on the physical, chemical, and sensory properties of strawberry flavored ice cream, European Food Research and Technology, 221: 466-470.
El-Nagar, G., Clowes, G., Tudorica, C.M., Kuri, V. & Brennan, C.S. 2002. Rheological quality and stability of yog-ice cream with added inulin. Dairy Technology, 55: 89-93.
Faergemand, M., Murray, B.S., Dickinson, E. & Qvist, Q.B. 1999. Cross-linking of adsorbed casein fims with transglutaminase. International Dairy, 9: 343-346.
Friedeck, K.G. & Karagul-Yuceer, Y. 2003. Soy protein fortification of a low –fat dairy- based ice cream. Food Science and Technology, 68: 2651-2657.
Gauche, C., Vieira, J.T.C., Ogliari, P.J. & Bordignon-Luiz, M.T. 2008. Crosslinking of milk whey proteins by transglutaminase. Process Biochemistry, 43: 788-794.
Gopalu, N.P., Rao, T.J., Ali, M.P. & Sastri, P.M. 1986. Effect of utilization of whey in ice cream. Dairy Science, 39: 94-95.
Hanson, A. P. & Heinis, J. J. 1991. Decrease of vanillin flavor perception in the presence of casein and whey proteins. Dairy Science, 74: 2936 –2940.
Hinz, K., Huppertz, T., Kulozik, U. & Kelly, A.L. 2007. Influence of enzymatic crosslinking on milk fat globules and emulsifying properties of milk proteins. International Dairy Journal, 17: 289- 293.
Jong, G. A. H. & Koppelman, S. J. 2002. Transglutaminase catalyzed reactions: impact on food applications. Food Science and Technology, 67: 2798- 2806.
Khalfalla, S. M. & Mahran, G. A. 1975. The Use of whey solids in ice cream. Egyptain Journal of Dairy Science, 3: 43-50.
Karaca, O.B., Guven, M., Yasar, K. & Kahyaoghlu, T. 2009. The functional, rheological and sensory characteristics of ice creams whit various fat replacers. Dairy Technology, 62: 93-99.
Kuraishi, C., Yamazaki, K. & Susa, Y. 2001. Transglutaminase: its utilization in the food industry. Food Reviews International, 17 (2): 221-246.
Lee, F.Y. & White, C.H. 1991. Effect of ultrafiltration retentates and whey protein concentrates on ice cream quality during storage. Dairy Science, 74: 1170–1180.
Lorenzen, P.C. 2000. Renneting properties of transglutaminase-treated milk. Milchwissenschaft, 55: 433–437.
Marshall, R. T. & Arbuckle, W.S. 1996. Ice cream. New York: Chapman and Hall.
Ohem, R.I., Marshal, R.T. & Heymann, H. 1997. Sensory and physical properties of ice cream containing milk fat or fat replacers. Dairy Science, 81: 1222-1228.
Pinterits, A. & Arntfield, S.D. 2008. Improvement of canola protein gelationproperties through enzymatic modifi cation with transglutaminase. LWT-Food Science and Technology, 41:128 138.
Rossa, N.R., Sá, E.M.F., Burin, V.M., & Bordignon-Luiz, M.T. 2011. Optimization of microbial transglutaminase activity in ice cream using response surface methodology. LWT-Food Science and Technology, 44 (1): 29-34.
Rossa, P.N., Burin, V.M. & Bordignon, T. 2012. Effect of microbial transglutaminase on functional and rheological properties of ice cream with different fat contents. LWT-Food Science and Technology, 48 (2): 224-230.
Ruger, P.R., Baer, R.J. & Kasperson, K.M. 2002. Effect of double homogenization and whey protein concentrate on the texture of ice cream. Dairy Science, 85: 1684–1692.
Thampson, L.U., Reniers, D.J., Baker, L.M. & Siu, M. 1983. Succinylated whey protein concentrates in ice cream and instant puddings. Dairy Science, 66: 1630-1637.
Wilcox, C.P. & Swaisgood, H.E. 2002. Modification of the rheological properties of whey protein isolate through the use of an immobilized transglutaminase. Agricultural and Food Chemistry, 50: 5546–51.
Yilsay, T.O., Yilmaz, L. & Bayzit, A.A. 2006. The effect of using a whey protein fat replacer on textural and sensory characteristics of low-fat vanilla ice cream. European Food Research Technology, 222: 171-175.
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Volume 3, Issue 3
October 2014
Pages 227-244
  • Receive Date: 09 April 2014
  • Revise Date: 03 October 2014
  • Accept Date: 12 October 2014