Effect of different microwave-extraction methods on active components and antimicrobial activities of lemon verbena essential oils

Golmakani, Mohammad-Taghi; Ghassemi, Armin; Eskandari, Mohammad Hadi; Niakosari, Mehrdad

doi:10.22101/JRIFST.2016.06.01.518

Effect of different microwave-extraction methods on active components and antimicrobial activities of lemon verbena essential oils

Document Type : Original Paper

Authors

¹ Assistant Professor, Department of Food Science and Technology, School of Agriculture, Shiraz University, Iran

² M.Sc. Graduated Student, Department of Food Science and Technology, School of Agriculture, Shiraz University, Iran

³ Associate Professor, Department of Food Science and Technology, School of Agriculture, Shiraz University, Iran

10.22101/JRIFST.2016.06.01.518

Abstract

In this study, effects of different extraction methods, namely microwave-assisted hydrodistillation (MAHD) and solvent-free microwave extraction (SFME) were investigated in comparison with that of conventional hydrodistillation (HD) in terms of yield, active components, and antimicrobial activities (4 Gram-positive bacteria and 4 Gram-negative bacteria) of lemon verbena leaves essential oils (EOs). Gas chromatography/ mass spectrometry results indicated that geranial (25.54-28.53 %), neral (20.76-23.51 %), and limonene (11.21-12.51 %) were main active components of lemon verbena EOs. There were significant differences among active components of EO obtained by SFME method with those of HD and MAHD methods (P<0.05). Antimicrobial activity of lemon verbena EO obtained by SFME method was higher than those of HD and MAHD methods, which is due to its higher oxygenated components such as geranial (increased by 4.96-11.70%) and neral (increased by 4.09-13.24%). Hence, SFME method can be proposed as a fast, efficient, and selective alternative method for extraction of EO from medicinal plants.

Keywords

References

اجاق، س. م.، رضایی، م.، رضوی، س. ه. و حسینی، س. م. ه. 1391. مطالعه اثر ضد باکتریایی اسانس پوست دارچین (CinnamomumZeylanicum) در شرایط آزمایشگاهی در برابر پنج باکتری عامل فساد غذایی. فصلنامه علوم و صنایع غذایی، 35: 76-67.

برومند، ع.، حامدی، م.، امام جمعه، ز.، رضوی، س. ه. و گلمکانی، م. ت. 1387. بررسی خاصیت میکروبی اسانس بذرهای شوید (Anethum garaveolens)، گشنیز (Coriangrum Sativum) بر روی استافیلوکوکوس اورئوس، اشرشیاکلی 0157:H7، سالمونلا تیفی موریوم با استفاده از آزمایش حساسیت رقت در محیط مایع. مجله پژوهش‌های علوم و صنایع غذایی ایران، 1: 68-59.

شاه حسینی، ر.، قربانی، ح.، صالح، ر. و امیدبیگی، ر. 1390. بررسی صفات کمی و کیفی اسانس بذر به لیمو (Lippiacitriodora). مجله پژوهش‌های تولید گیاهی، 18: 96-91.

مظفریان، و. 1394. درختان و درختچه‌های ایران. انتشارات فرهنگ معاصر، تهران، صفحه 1050.

AACC. 1983. Approved methods of the American Association of Cereal Chemists. 8th ed. Salem, MA: Library of Congress.

Ali, H.F.M., El-Beltagi, H.S., & Nasr, N.F. 2011. Evaluation of antioxidant and antimicrobial activity of Aloysia triphylla. Electronic Journal of Environmental, Agricultural and Food Chemistry, 10:2689-2699.

Andrews, J.M. 2001. Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 48:5-16.

Fellows, P.J. 2000. Food Processing Technology, 2nd Ed., CRC Press Inc, New York.

Ferhat, M.A., Meklati, B.Y., Smadja, J., & Chemat, F. 2006. An improved microwave clevenger apparatus for distillation of essential oils from orange peel. Journal of Chromatography A, 1112:121-126.

Golmakani, M.T., & Moayyedi, M. 2015. Comparison of heat and mass transfer of different microwave-assisted extraction methods of essential oil from Citrus limon (Lisbon variety) peel. Food Science & Nutrition, 3:506-518.

Golmakani, M.T., & Rezaei, K. 2008a. Comparison of microwave-assisted hydrodistillation with the traditional hydrodistillation method in the extraction of essential oils from Thymus vulgaris L. Food Chemistry, 109:925-930.

Golmakani, M.T., & Rezaei, K. 2008b. Microwave-assisted hydrodistillation of essential oils from Zataria multiflora Boiss. European Journal of Lipid Science and Technology, 110:448-454.

Karakaya, S., El, S.N., Karagozlu, N., Sahin, S., Sumnu, G., & Bayramoglu, B. 2012. Microwave-assisted hydrodistillation of essential oil from Rosemary. Journal of Food Science and Technology, 51:1056-1065.

Lira, P.D.L., van Baren, C.M., Retta, D., Bandoni, A.L., Gil, A., Gattuso, M., & Gattuso, S. 2008. Characterization of Lemon Verbena (Aloysia citriodora Palau) from Argentina by the Essential Oil. Journal of Essential Oil Research, 20(4):350-353.

Mazidi, S., Rezaei, K., Golmakani, M.-T., Sharifan, A., & Rezazadeh, Sh. 2012. Antioxidant activity of essential oil from Black Zira (Bunium persicum Boiss.) obtained by microwave-assisted hydrodistillation. Journal of Agricultural Science and Technology, 14:1013-1022.

Okoh, O.O., Sadimenko, A.P., & Afolayan, A.J. 2010. Comparative evaluation of the antibacterial activities of the essential oils of Rosmarinus officinalis L. obtained by hydrodistillation and solvent-free microwave extraction methods. Food Chemistry, 120:308-312.

Pascual, M.E., Slowing, K., Carretero, E., Sanchez, M.D., & Villar, A. 2001. Lippia: traditional uses, chemistry and pharmacology: a review. Journal of Ethnopharmacology, 76:201-214.

Qi, X.-L., Li, T.-T., Wei, Z.-F., Guo, Na., Luo, M., Wang, W., Zu, Y.G., Fu, Y.J., & Peng, X. 2014. Solvent-free microwave extraction of essential oil from pigeon pea leaves [Cajanus cajan (L.) Millsp.] and evaluation of its antimicrobial activity. Industrial Crops and Products, 58:322-328.

Rezvanpanah, S., Rezaei, K., Golmakani, M.-T., & Razavi, S.H. 2011. Antibacterial properties and chemical characterization of the essential oils from summer savory extracted by microwave-assisted hydrodistillation. Brazilian Journal of Microbiology, 42:1453-1462.

Shareef, A.A. 2011. Evaluation of Antibacterial activity of essential oils of Cinnamomum sp. and Boswellia sp. Journal of Basrah Researches (Sciences), 37(5A):60-71.

Uysal, B., Sozmen, F., Kose, E.O., Gokhan Deniz, I., & Oksal, B.S. 2010. Solvent-free microwave extraction and hydrodistillation of essential oils from endemic Origanum husnucanbaseri H. Duman, Aytac & A. Duran: comparison of antibacterial activity and contents. Natural Product Research, 24:1654-1663.

Velazquez, C., Calzada, F., Torres, J., Gonzalez, F., & Ceballos, G. 2006. Antisecretory activity of plants used to treat gastrointestinal disorders in Mexico. Journal of Ethnopharmacology, 103: 66-70.

Wang, L., & Weller, L.C. 2006. Recent advances in extraction of nutraceuticals from plants. Trends in Food Science and Technology, 17:300-312.

Xiao, X., Song, W., Wang, J., & Li, G. 2012. Microwave-assisted extraction performed in low temperature and in vacuo for the extraction of labile compounds in food samples. Analytica Chimica Acta, 712:85-93.