Immobilization of Saccharomyces Cerevisiae on Alumina Ceramic Beads In Order to Reduce Aflatoxin M1 In Vitro

Foroughi, Marjan; Sarabi Jamab, Mahbobeh; Keramat, Javad; Najaf Najafi, Masoud

doi:10.22101/JRIFST.2016.12.19.538

Immobilization of Saccharomyces Cerevisiae on Alumina Ceramic Beads In Order to Reduce Aflatoxin M1 In Vitro

Document Type : Original Paper

Authors

¹ PhD. Student, Department of Food Biotechnology, Research Institute of Food Science and Technology, Mashhad, Iran

² Assistant Professor, Department of Food Biotechnology, Research Institute of Food Science and Technology, Mashhad, Iran

³ Associated Professor, Department of Food Science and Technology, College of Agricultural, Isfahan, Iran

⁴ Assistant Professor, Institute of Scientific-Applied Higher Education Jihad-e-Agriculture, Mashhad, Iran

10.22101/JRIFST.2016.12.19.538

Abstract

In this study the ability of Saccharomyces cerevisiae PTCC 5052 to adsorption of aflatoxin M₁ was assessed. In order to improve operational efficiency, Alumina ceramic was analyzed as a potential support for immobilization of yeast cells. In immobilization process, results indicated that the binding abilities of AFM₁ by live cells of Saccharomyces cerevisiae were higher than non-live immobilized cells on alumina ceramic within 48 hours (P<0.05). Then, the aflatoxin M₁ solution (0.2 ppb) was passed through a ceramic substrate containing immobilized saccharomyces cerevisiae (both of live and non-live immobilized cells) in 5, 10 and 20 minutes. The results showed that the residual aflatoxin M₁ in the solution after 20 minutes of circulation was minimal and the highest percentage of AFM₁ reduction was 75. Alumina beads containing live immobilized yeast cells compared non- live yeasts, significantly reduced aflatoxin M_1.The results of this study showed that the alumina ceramic can be used as a suitable bed for immobilization of saccharomyces cerevisiae to remove aflatoxin M_1.

Keywords

References

سازمان ملی استاندارد ایران. 1388. خوراک انسان دام، بیشینه رواداری مایکوتوکسین‌ها ، استاندارد ملی ایران، شماره 5925، اصلاحیه.

سازمان ملی استاندارد ایران. 1390. شیر و فراورده‌های آن، اندازه‌گیری آفلاتوکسین M1 به روش کروماتوگرافی مایع با کارایی بالا، استاندارد ملی ایران، شماره 7133، تجدیدنظر اول.

Azab, R.M., Tawakkol, W.M., Hamad, A.R.M., Abou-Elmagd, M.K., El -Agrab, H.M., & Refai, M.K. 2005. Detection and estimation of aflatoxin B1 in feeds and its biodegradation by bacteria and fungi. Egypt Journal of Natural Toxins, 2:20-39.

Beshay, U., Hesham, E., Ismail, I., & Moavad, H. 2011. β-glucanase by a recombinant strain of Escherichia coli immobilized in different matrices. Polish Journal of Microbiology, 2:133–138.

Corassin, C., Bovo, F., Rosim, H., & Oliveira, R. E. 2012. Efficiency of Saccharomyces cerevisiae and lactic acid bacteria strains to bind aflatoxin M1 in UHT skim milk. Food Control, 31:80-83.

Elgerbi, A.M., Aidoo, K.E., Candlish, A.A.G., & Williams. A. G. 2006. Effects of lactic acid bacteria and bifidobacteria on levels of aflatoxin M1 in milk and phosphate‌ buffer. Milch wissenschaft, 61(2):197-199.

Fallah, a., jafari, T., Fallah, A., & Rahnama, M., 2009. Determination of aflatoxin M1 levels in Iranian white and cream cheese. Food and Chemical Toxicology, 47 (8):1872-1873.

Hamdy, M., Kim, K.K., & Rudtke, C.A. 1990. Continuous ethanol production by yeast immobilized on to channeled Alumina beads .journal of Biomass, 21:189-206.

Janiszyn,z ., Dziuba, E., Boruczkowski, T., Chmielewsk, J., Kawa-Rygielska, J., & Rosiek, G. 2007. Ethanol fermentation with yeast cell immobilized on grains of porous ceramic sinter. Polish Journal of Food and Nutrition Sciences, 57:245-250.

Kabak, B., & Var, I. 2008. Factors affecting the removal of aflatoxin M1 from food model by Lactobacillus and Bifidobacterium strains. Journal of Environmental Science and Health B. 43 (7):-617-624.

karimi, A. 2009. Decolorization of Maxilon-Red by Kissiris Immobilized Phanerochaete Chrysosporium in a Trickle-Bed Bioreactor-Involvement of Ligninolytic Enzymes

Kourkoutas,Y., Bekatoroua, A., Banat, I.M., Marchantb, R., & Koutinas, A.A. 2004. Immobilization technologies and support materials suitable in alcohol beverages production: a review. Food Microbiology, 21:377–39.

Moss, M. O. 1998. Economic importance of mycotoxins. J. Appl. Microbiol, 84:62–76.

Navarro, j.m., & Durand, G. 1997. Modification of Yeast Metabolism by Immobilization on to Porous Glass. European Journal of Applied Microbiology, 4:243-254.

Oveisi, M.R., Janat, B., sadeghi,N., Hajimahmoodi, & M., Nikzad,a., 2007. Presence of aflatoxin M1 in milk and infant milk products in Tehran, Iran. Food Control, 18 (10):1216–1218.

Pereira, A.P., Mendes-Ferreira, A., Oliveira, J.M., Estevinho, L.M., & Mendes, A. 2013. Effect of Saccharomyces cerevisiae cells immobilization on mead Production. Journal of Food Science and Technology, 56:21-30.

Rapoport, A,. Borovikova, D., Kokina, A., Patmalnieks, A., Polyak, N., Pavlovska, L., Mezinskis, G., & Dekhtyar, Y., 2011. Immobilization of yeast cells on the surface of hydroxyapatite ceramics Process Biochemistry, 46:665–670.

Razzaghi-Abyaneh, M. 2013. Aflatoxins , Recent Advances and Future Prospects, Iva Simcic, http://dx.doi.org/10.57772/2500.

Rahaie, S., Emam-jomeh, Z.S., Razavi1, H., & Mazaheri, M. 2010. Immobilized Saccharomyces Cerevisiae as a potential Aflatoxin decontamination agent in Pistachio nuts. Brazilian Journal of Microbiology, 41:82-90.

Shahin, A.A.M. 2007. Removal of aflatoxin B1 from contaminated liquid media by dairy lactic acid bacteria. International Journal of Agriculture and Biology, 9(1):71-75.

Shetty, P.H. & Jespersen, L. 2006. Saccharomyces cervisiae and lactic acid bacteria as potential mycotoxin decontaminating agents. Trends in Food Science and Technology, 17:48-55.

Webber, A., Hettiarachchy, N.S., webber, D., Sivarooban, T., & Horax, R. 2014. Heat-Stabilized Defatted Rice Bran (HDRB) as an Alternative Growth Medium for Saccharomyces cerevisiae. Journal of Food and Nutrition, 1:1-6.