Factors Affecting the Production and Stability of Optimal Formulation of Nanoemulsion Containing Vitamin A and D by Spontaneous Production

Document Type : Original Paper

Authors

1 Assistant Professor of Department of Food Science and Technology, Taculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Instructor of Department of Food Science and Technology of Samin Nan Sahar, Tehran, Iran

3 MS Graduated of Department of Food Science and Technology, Taculty of Agriculture, University of Tabriz, Tabriz, Iran

4 Department of Food Science and Technology, Faculty of Agriculture, Azad University of Shahriar, Shahrak Ghods, Tehran, Iran

5 Instructor of Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

Abstract

Due to their unique functional properties, using nanoemulsions for the enrichment of many hydrophobic compounds in beverages is beneficial. In this study, vitamins A and D nano emulsions produced by spontaneous formation using various surfactants (tween 60, 80 and 85) and corn oil and miglyol 812 as carrier oils; various types of vitamins A and D (2:1, 1:1 and 1:2) and various surfactant emulsion ratios (SER). Measurement of the average diameter of the droplets, zeta potential, rheology, turbidity and stability of system during storage time were done. Analysis of the results by laser light dispersion in completely randomized design showed the particle size in formulation with SER=17.5%, using tween 80 and miglyol 812 including 5% vitamin A and D, was equal to 81 nm with -27.9 mv zeta potential was gained. The optimum sample was stable at a temperature of 23 °C during storage time (3 months). The images of the electron microscopy also confirmed the results of measuring the size of droplets by particle size analyzer. The study of the rheological behavior of optimum nano emulsion also indicated the Newtonian behavior and the non-dependence viscosity by shear-shear rates.

Keywords

Anton, N., & Vandamm, T. (2009, September). Low energy nano- emulsificasions: overview and potentials in microencapsulation, (pp. 1-4). XVII International Conference on Bioencapsulation, Groningen, Netherland.
Champagne, C.P., & Fustier, P. (2007). Microencapsulation for the improved delivery of bioactive compounds into foods. Current Opinion in Biotechnology, 18(2), 184-190. doi: https://doi.org/10.1016/j.copbio.2007.03.001
Davidov-Pardo, G., & McClements, D.J. (2014). Resveratrol encapsulation: designing delivery systems to overcome solubility, stability and bioavailability issues. Trends in Food Science & Technology, 38(2), 88-103. doi:https://doi.org/10.1016/j.tifs.2014.05.003
Famian, H. (2016). Enrichment of low-fat pasteurized milk by conjugated linoleic acid nano emulsion. (Unpublished master's thesis),, Department of Food Science and Technology, Islamic Azad University, Mamaghan Branch. (in Persian)
Fathi, M., Mozafari M.R., & Mohebbi, M. (2012). Nanoencapsulation of food ingredients using lipid based delivery systems. Trends in Food Science & Technology, 23(1), 13-27. doi:https://doi.org/10.1016/j.tifs.2011.08.003
Fatouros, D.G., & Antimisiaris, S.G. (2002). Effect of amphiphilic drugs on the stability and zeta-potential of their liposome formulations: a study with prednisolone, diazepam, and griseofulvin. Journal of Colloid and Interface Science, 251(2), 271-277. doi:https://doi.org/10.1006/jcis.2002.8432
Ghanbarzadeh, B. (2013). Handbook of Physics chemistry and physical of colloidal systems and biopolymer of Food. Academic publications of Industrial Sharif Uniniversity. (in Persian)
Ghanbarzadeh, B., Babazadeh, A., & Hamishekar, H. (2016). Nano-phytosome as a potential food-grade delivery system. Food Bioscience, 15, 126-135. doi:https://doi.org/10.1016/j.fbio.2016.07.006
Gonnet, M., Lethuaut, L., & Boury, F. (2010). New trends in encapsulation of liposoluble vitamins. Journal of Controlled Release, 146(3), 276-290. doi:https://doi.org/10.1016/j.jconrel.2010.01.037
Guttoff, M., Saberi, A.H., & McClements, D.J. (2015). Formation of vitamin D nanoemulsion-based delivery systems by spontaneous emulsification: factors affecting particle size and stability. Food Chemistry, 171, 117-122. doi:https://doi.org/10.1016/j.foodchem.2014.08.087
Hasani, F. (2015). Production and optimization of nanoemulsion formulation containing beta carotene (by spontaneous emulsification method). (Unpublished master's thesis), Department of Food Science and Technology, Islamic Azad University, Mamaghan Branch. (in Persian)
Klang, V., Matsko, N.B., Valenta, C., & Hofer, F. (2012). Electron microscopy of nanoemulsions: an essential tool for characterisation and stability assessment. Micron, 43(2-3), 85-103. doi:https://doi.org/10.1016/j.micron.2011.07.014
Komaiko, J., & McClements, D.J. (2014). Optimization of isothermal low-energy nanoemulsion formation: Hydrocarbon oil, non-ionic surfactant, and water systems. Journal of Colloidal and Interface Science, 425, 59-66. doi:https://doi.org/10.1016/j.jcis.2014.03.035
Li, Y., Zheng, J., Xiao, H., & McClements, D.J. (2012). Nanoemulsion-based delivery systems for poorly water-soluble bioactive compounds: Influence of formulation parameters on polymethoxyflavone crystallization. Food Hydrocolloids, 27(2), 17-528. doi:https://doi.org/10.1016/j.foodhyd.2011.08.017
Masah, M., Ghanbarzadeh, B., Hamishekar, H., & Sowti Khiabani, M. (2013, October). The effect of oil phase composition on the production of nanoemulsions containing alfatucophorol using low energy method. 21st National Congress of Food Science and Technology, Shiraz. (in Persian)
McClements, D.J. (2005). Food Emulsions: Principles, Practice and Technology. CRC Press: Boca Raton, Florida.
Najafi-Taher, R., & Amani, A. (2017). Nanoemulsions: colloidal topical delivery systems for antiacne agents- a mini-review. Nanomedicine Research Journal, 2(1), 49-56. doi:https:/doi.org/10.22034/NMRJ.2017.23532
Niknia N., Ghanbarzadeh B., Hamishekar H., Rezayi Mokarramm R., & Mortazaviyan, A. (2014). Production and Evaluation of Vitamin E based Nanoemulsion by Spontaneous Method. Iranian Journal of Nutrition Sciences & Food Technology, 8(4), 51-65. (in Persian)
Ostertag, T., Weiss, J., & McClements, D.J. (2012). Low-energy formation of edible nanoemulsions: factors influencing droplet size produced by emulsion phase inversion. Journal of Colloid and Interface Science, 388(1), 95-102. doi:https://doi.org/10.1016/j.jcis.2012.07.089
Pezeshki, A., Ghanbarzadeh, B., Hamishehkar, H., Moghadam, M., & Fathollahi, I. (2016). Vitamin A palimitate-loaded nanoemulsions produced by spontaneous emulsification method: effect of surfactant and oil on droplet size and stability. Journal of Research and Innovation in Food Science and Technology, 4(4), 299-314. doi:http://dx.doi.org/10.22101/jrifst.2016.01.30.442 (in Persian)
Rao, R., & McClements, D.J. (2012). Food-grade microemulsions and nanoemulsions: role of oil phase composition on formation and stability. Food Hydrocolloids, 29(2), 326-334. doi:https://doi.org/10.1016/j.foodhyd.2012.04.008
Saberi, A.H., Fang, Y., & McClements, D.J. (2013). Fabrication of vitamin E-enriched nanoemulsions: factors affecting particle size using spontaneous emulsification. Journal of Colloid and Interface Science, 391, 95-102. doi:https://doi.org/10.1016/j.jcis.2012.08.069
Salager, J.L., Forgiarini, A., Marquez, L., Pena, A., Pizzino, A., Rodriguez, M.P., & Rondon-Gonzalez, M. (2004). Using emulsion inversion in industrial processes. Advances in Colloid Interface Science, 108-109, 259-272. doi:https://doi.org/10.1016/j.cis.2003.10.008
Silva, H.D., Cerqueira, M.A., & Vicente, A.A. (2012). Nanoemulsions for food applications: development and characterization. Food and Bioprocess Technology, 5(3), 854-867. doi:http://dx.doi.org/10.1007/s11947-011-0683-7
 Sood, S., & Gowthamarajan, KJ.K. (2014). Optimization of curcumin nanoemulsion for intranasal delivery using design of experiment and its toxicity assessment. Colloids and Surfaces B: Biointerfaces, 113, 330-337. doi:https://doi.org/10.1016/j.colsurfb.2013.09.030
Wulff-Perez, M., Torcello-Gomez, A., Galvez-Ruiz, M.J., & Rodrıguez-Martin, A. (2009). Stability of emulsions for parenteral feeding: Preparation and characterization of o/w nanoemulsions with natural oils and Pluronic f68 as surfactant. Food Hydrocolloids, 23(4), 1096-1102. doi:https://doi.org/10.1016/j.foodhyd.2008.09.017
Yang, Y., Leser, M.E., Sher, A.A., & McClements, D.J. (2013). Formation and stability of emulsions using a natural small molecule surfactant: quillaja saponin (q-naturaleÒ). Food Hydrocolloids, 30(2), 589-596. doi:https://doi.org/10.1016/j.foodhyd.2012.08.008
Yang, Y., Marshall-Breton, C., Leser, M.E., Sher, A.A., & McClements, D.J. (2012). Fabrication of ultrafine edible emulsions: comparison of high-energy and low-energy homogenization methods. Food Hydrocolloids, 29(2), 398-406. doi:https://doi.org/10.1016/j.foodhyd.2012.04.009
CAPTCHA Image
Volume 7, Issue 3
October 2018
Pages 255-268
  • Receive Date: 14 September 2017
  • Revise Date: 23 February 2018
  • Accept Date: 25 April 2018