A Study on the Qualitative Characteristics and Microbial Survival of Lactobacillus acidophilus and Bacillus coagulans in Probiotic Bread

Hosseininezhad, Marzieh; Abedfar, Abbas

doi:10.22101/JRIFST.2018.10.20.738

A Study on the Qualitative Characteristics and Microbial Survival of Lactobacillus acidophilus and Bacillus coagulans in Probiotic Bread

Document Type : Original Paper

Authors

¹ Associate Professor, Department of Food Biotechnology, Research Institute of Food Science and Technology, Mashhad, Iran

² Ph.D Candidate, Department of Food Biotechnology, Research Institute of Food Science and Technology, Mashhad, Iran

10.22101/JRIFST.2018.10.20.738

Abstract

Nowadays, the role of nutrition in the health of gastrointestinal system is deemed as a key factor in the production of functional foods with probiotic bacteria especially in the bakery industry. Therefore, microencapsulation of probiotics and their survival during baking and gastrointestinal aggregation have positive physiology effects on consumers. The aim of the research was to study qualitative characteristics and microbial survival of probiotic bread produced with 1, 1.5 and 2% encapsulated microbial suspension along with edible starch 5% during 48 h. The results of this assessment showed that the time of storage reduced microbial population of the probiotic bread in such way that the population decline in the 1% suspension was more severe. Also, in both samples containing different microbial suspensions, the specific volume decreased while the porosity increased within 48 (P≤ 0.05), compared to the control. Whereas, hardness of probiotic bread increased after of 24 h which was less than the control sample (P≤0.05). Moreover, the analysis of variance and mean comparison of colorimetric test showed a significant effect (P≤ 0.05) on the color indexes of (L^*, a^* and b^*) after 48 hours.

Keywords

References

AACC International. (2010). AACC methods (11^th Ed): Approved methods of the American Association ofCereal Chemists.(pp. 46-30,): The St. Paul.

Altamirano-Fortoul, R., Moreno-Terrazas, R., Quezada-Gallo, A., & Rosell, C.M. (2012). Viability of some probiotic coatings in bread and its effect on the crust mechanical properties. Food Hydrocolloids, 29(1), 166-174. doi:https://doi.org/10.1016/j.foodhyd.2012.02.015

Ananta, E., Volkert, M., & Knorr, D. (2005). Cellular injuries and storage stability of spray-dried lactobacillus rhamnosus GG. International Dairy Journal, 15(4), 399-409. doi:https://doi.org/10.1016/j.idairyj.2004.08.004

de Conto, L. C., Oliveira, R. S. P., Martin, L. G. P., Chang, Y. K., & Steel, C. J. (2012). Effects of the addition of microencapsulated omega-3 and rosemary extract on the technological and sensory quality of white pan bread. LWT-Food Science and Technology, 45(1), 103-109. doi:https://doi.org/10.1016/j.lwt.2011.07.027

Doherty, S.B., Gee, V.L., Ross, R.P., Stanton, C., Fitzgerald, G.F., & Brodkorb, A. (2010). Efficacy of whey protein gel networks as potential viability-enhancing scaffolds for cell immobilization of Lactobacillus rhamnosus GG. Journal of Microbiological Methods, 80(3), 231-241. doi:https://doi.org/10.1016/j.mimet.2009.12.009

Ehtiati, A., Mohebbi, M., & Shahidi, F. (2008, October). Application of Image Processing in colorimetry of Bread crust. 18^th National Congress on Food science and Industries, Mashhad. https://www.civilica.com/Paper-NCFOODI18-NCFOODI18_045.html (in Persian)

Fu, N., & Chen, X.D. (2011). Towards a maximal cell survival in convective thermal drying processes. Food Research International, 44(5), 1127-1149. doi:https://doi.org/10.1016/j.foodres.2011.03.053

Guarda, A., Rosell, C.M., Benedito, C., & Galotto, M.J. (2004). Different hydrocolloids as bread improvers and antistaling agents. Food hydrocolloids, 18(2), 241-247. doi:https://doi.org/10.1016/S0268-005X(03)00080-8

Haralick, R.M., Shanmugam, K., & Dinstein, I. (1973). Textural features for image classification. IEEE Transactions on Systems, Man and Cybernetics, 3(6), 610-621. doi:https://doi.org/10.1109/TSMC.1973.4309314

Homayouni, A., Ehsani, M.R., Azizi, A., Yarmand, M.S., & Razavi, S.H. (2007). Effect of lecithin and calcium chloride solution on the microencapsulation process yield of calcium alginate beads. Iranian Polymer Journal. 16(9), 597-606.

Hosseininezhad, M., Anvari, H., Zhiani, M., & Abedfar, A. (2017). Evaluating the effect of inulin supplementary on the sensory and textural properties of prebiotic bread (Taftoon). Journal of Research and Innovation in Food Science and Technology, 6(2), 185-198. doi:http://dx.doi.org/10.22101/jrifst.2017.09.02.626 (in Persian)

Hossieninezhad, M., Hussain, M.A. & Britz, M.L. (2015). Stress response in probiotic Lactobacillus casei. Critical Reviews in Food Science and Nutrition. 55(6), 740-749. doi:https://doi.org/10.1080/10408398.2012.675601

Iranian National Standardization Organization. (2003). Cereal and cereal products-Flat bread-Barbary-Code of practice. (ISIRI Standard No. 5809, 1^st. Revision). Retrieved from http://standard.isiri.gov.ir/StandardView.aspx?Id=3666 (in Persian)

Jackman, R.L., & Stanley, D.W. (1992). Area-and perimeter-dependent properties and failure of mature-green and red-ripe tomato pericarp tissue. Journal of Texture Studies, 23(4), 461-474. doi:https://doi.org/10.1111/j.1745-4603.1992.tb00034.x

Kosin, B., & Rakshit, S.K. (2010). Induction of heat tolerance in autochthonous and allochthonous thermotolerant probiotics for application to white shrimp feed. Aquaculture, 306(1-4), 302-309. doi:https://doi.org/10.1016/j.aquaculture.2010.04.017

Majzoobi, M., Farahnaky, A., Nematolahi, Z., Mohamadi Hashemi, M., & Taghipour Ardakani, M.J. (2013). Effect of different levels and particle sizes of wheat bran on the quality of flat bread. Journal of Agricultural Science and Technology, 15(1), 115-123.

Mokarram, R.R., Mortazavi, S.A., Habibi Najafi, M.B., & Shahidi, F. (2009). The influence of multi stage alginate coating on survivability of potential probiotic bacteria in simulated gastric and intestinal juice. Food Research International, 42(8), 1040-1045. doi:https://doi.org/10.1016/j.foodres.2009.04.023

Razavizadegan jahromi, S.M., Karimi, M., Tabatabaee yazdi, F., & Mortazavi, S.A. (2013). Response surface optimization of barbari bread-making process variables: interrelationship of texture, image and organoleptic characteristics; using image analysis for quality and shelf life prediction. Journal of Food Processing and Preservation, 38(4), 1608-1621. doi:https://doi.org/10.1111/jfpp.12123

Rokka, S., & Rantamäki, P. (2010). Protecting probiotic bacteria by microencapsulation: challenges for industrial applications. European Food Research and Technology, 231(1), 1-12. doi:https://doi.org/10.1007/s00217-010-1246-2

Saarela, M., Mogensen, G., Fonden, R., Matto, J., & Mattila-Sandholm, T. (2000). Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology, 84(3), 197-215. doi:https://doi.org/10.1016/S0168-1656(00)00375-8

Salehi, F., & Kashaninejad, M. (2015). Effect of drying methods on rheological and textural properties, and color changes of wild sage seed gum. Journal of Food Science and Technology, 52(11), 7361-7368. doi:https://doi.org/10.1007/s13197-015-1849-5

Seyedain-Ardabili, M., Sharifan, A., & Ghiassi Tarzi, B. (2016). The production of synbiotic bread by microencapsulation. Food Technology and Biotechnology, 54(1), 52-59. doi:https://doi.org/10.17113/ftb.54.01.16.4234

Shafiee, S., Motlagh, M.A., Didar, A.R., & Minaee, S. (2008). Investigation the effect of skin on mechanical behavior of apple. Journal of Food Technology, 6(2), 86-91.

Shehzad, A., Chiron, H., Della Valle, G., Kansou, K., Ndiaye, A., & Reguerre, A.L. (2010). Porosity and stability of bread dough during proofing determined by video image analysis for different compositions and mixing conditions. Food Research International, 43(8), 1999-2005. doi:https://doi.org/10.1016/j.foodres.2010.05.019

Wu, Z., He, Y., Chen, L., Han, Y., & Li, C. (2014). Characterization of Raoultella planticola Rs-2 microcapsule prepared with a blend of alginate and starch and its release behavior. Carbohydrate Polymer, 110, 259-267. doi:https://doi.org/10.1016/j.carbpol.2014.04.011

Yousefi, H., Soleimanian-Zad, S., & Shahedi Bagh Khandan, M. (2016). Microencapsulation of probiotics by emulsion method for production of probiotic bread. Iranian Journal of Nutrition Sciences & Food Technology, 4(11), 99-106. (in Persian)

Zhang, L., Huang, S., Ananingsih, V.K., Zhou, W., & Chen, X.D. (2014). A study on bifidobacterium lactis Bb12 viability in bread during baking. Journal of Food Engineering, 122, 33-37. doi:https://doi.org/10.1016/j.jfoodeng.2013.08.029

Zheng, C., Sun, D.-W., & Zheng, L. (2006). Recent developments and applications of image features for food quality evaluation and inspection-a review. Trends of Food Science & Technology, 17(12), 642-655. doi:https://doi.org/10.1016/j.tifs.2006.06.005