Document Type : Original Paper


Assistant Professor, Department of Food Science and Engineering,, University of Zanjan, Zanjan, Iran


Fluid gels already proposed to deliver flavors and nutrients safely. In this paper, our earlier research was applied for mechanical, rheological and release evaluation of the vitamin encapsulated alginate-whey protein microcapsules. The results indicated that the size distribution of alginate-whey protein microcapsules depended on the whey protein concentration, alginate concentration, and emulsification method; the mean diameters of these microcapsules slightly increased as the whey protein and alginate concentration increased. The microcapsules containing riboflavin and biotin showed significant changes in mean diameter volume, but vitamin stability did not change during 30 days at 4 °C. The micro-gels emulsified by ultrasound exhibited a decrease in stiffness than those produced by high-shear blending. The vitamin encapsulated alginate-whey protein microcapsules emulsified by ultrasound were quite stable compared to microcapsule emulsified with the agitator. These micro-gel suspensions exhibit a fluid-like behavior. We founded that the release from these microcapsules mainly occurred by diffusion mechanisms. In summary, this research suggested that alginate-whey protein microcapsules can protect the active and bioactive agent against stomach condition. Our developed vesicular system could be used to nutrient delivery or controlled nutrient release.


Abbasi, F., Samadi, F., Jafari, S. M., Ramezanpour, S., & Shams Shargh, M. (2019). Ultrasound-assisted preparation of flaxseed oil nanoemulsions coated with alginate-whey protein for targeted delivery of omega-3 fatty acids into the lower sections of gastrointestinal tract to enrich broiler meat. Ultrasonics Sonochemistry, 50, 208-217. doi:
Aguilera, J. M., & Park, D. J. (2016). Texture-modified foods for the elderly: Status, technology and opportunities. Trends in Food Science & Technology, 57, 156-164. doi:
Barbosa, M. I. M. J., Borsarelli, C. D., & Mercadante, A. Z. (2005). Light stability of spray-dried bixin encapsulated with different edible polysaccharide preparations. Food Research International, 38(8), 989-994. doi:
Beck, W. (2001). Handbook of Vitamins, ; Rucker, RB, Suttie, JW, McCormick, DB, Machlin, LJ, Eds: Marcel Dekker: New York.
Chen, L., & Subirade, M. (2006). Alginate–whey protein granular microspheres as oral delivery vehicles for bioactive compounds. Biomaterials, 27(26), 4646-4654. doi:
Ching, S. H., Bansal, N., & Bhandari, B. (2015). Physical stability of emulsion encapsulated in alginate microgel particles by the impinging aerosol technique. Food Research International, 75, 182-193. doi:
Cichero, J. A. Y. (2016). Adjustment of Food Textural Properties for Elderly Patients. Journal of Texture Studies, 47(4), 277-283. doi:
Combs Jr, F. (2008). Vitamin C. In The vitamins. Fundamental aspects in nutrition and health/FG Jr. Combs. (Third edition ed.) (pp. 235-265): Amsterdam Boston: Elsevier Aca demic Press.
DESA, U. (2015). World population prospects: the 2015 revision, key findings and advance tables. United Nations Department of Economic and Social Affairs. Population Division working paper no ESA/P/WP, 241.
Ghahremanifar, A., Mohamadu Sani, A., Najaf Najafi, M., & Mohamadi moghadam, T. (2010). Effect of concentration and homogenization time on the emulsification of concentrate whey. Novelty in food science and technology, 2(2), 35-45. (in Persian)
Kim, K. H., Renkema, J. M. S., & van Vliet, T. (2001). Rheological properties of soybean protein isolate gels containing emulsion droplets. Food Hydrocolloids, 15(3), 295-302. doi:
Koocheki, A., Kadkhodaee, R., Mortazavi, S. A., Shahidi, F., & Taherian, A. R. (2009). Influence of Alyssum homolocarpum seed gum on the stability and flow properties of O/W emulsion prepared by high intensity ultrasound. Food Hydrocolloids, 23(8), 2416-2424. doi:
Leon, A. M., Aguilera, J. M., & Park, D. J. (2019). Mechanical, rheological and structural properties of fiber-containing microgels based on whey protein and alginate. Carbohydrate Polymers, 207, 571-579. doi:
Leon, A. M., Medina, W. T., Park, D. J., & Aguilera, J. M. (2018). Properties of microparticles from a whey protein isolate/alginate emulsion gel. Food Science and Technology International, 24(5), 414-423. doi:
Leon, A. M., Medina, W. T., Park, D. J., & Aguilera, J. M. (2016). Mechanical properties of whey protein/Na alginate gel microparticles. Journal of Food Engineering, 188, 1-7. doi:
Mohammadi, M., Ghanbarzadeh, B., Mokarram, R. R., Hoseini, M., & Hamishehkar, H. (2014). Study of Stability, Zeta-potential, and Steady Rheological Properties of Nanoliposomes Containing Vitamin D3. Medical Journal of Tabriz University of Medical Sciences and Health Services, 36(4), 102-111. (in Persian)
Nedovic, V., Kalusevic, A., Manojlovic, V., Levic, S., & Bugarski, B. (2011). An overview of encapsulation technologies for food applications. Procedia Food Science, 1, 1806-1815. doi:
O'Donnell, P. B., & McGinity, J. W. (1997). Preparation of microspheres by the solvent evaporation technique. Advanced Drug Delivery Reviews, 28(1), 25-42. doi:
Painter, V., Le Couteur, D. G., & Waite, L. M. (2017). Texture-modified food and fluids in dementia and residential aged care facilities. Clinical interventions in aging, 12, 1193-1203. doi:
Rosenberg, M., & Lee, S. J. (2004). Calcium-alginate coated, whey protein-based microspheres: preparation, some properties and opportunities. Journal of Microencapsulation, 21(3), 263-281. doi:
Sala, G., Van Aken, G. A., Stuart, M. A. C., & Van De Velde, F. (2007). Effect of droplet–matrix interactions on large deformation properties of emulsion-filled gels. Journal of Texture Studies, 38(4), 511-535. doi:
Zandi, M. (2019). Vitamin protection by Alginate-Whey Protein Micro Gel (AL-WPC MG) as a novel microcapsule against gastrointestinal condition; case study: B-complex vitamins. Iranian Food Science and Technology Research Journal, 16(3). doi:
Zandi, M. (2017). Evaluation of the Kinetics of Ascorbic Acid (AA) Release from Alginate-Whey Protein Concentrates (AL-WPC) Microspheres at the Simulated Gastro–Intestinal Condition. Journal of Food Process Engineering, 40(1), e12334. doi:
Zandi, M., Mohebbei, M., Varidi, M., & Ramezanian, N. (2015). Estimation and evaluation of diffusion coefficient of diacetyl encapsulated alginate-whey protein microspheres at simulated mouth conditions. Iranian Food Science and Technology Research Journal, 11(4), 435. (in Persian)
Zandi, M., & Mohebbei, M. (2014). Investigation of encapsulated diacetyl colloidosome release profile as a function of sintering process and release media properties. Flavour and Fragrance Journal, 29(6), 364-370. doi:
Zandi, M., Mohebbi, M., Varidi, M., & Ramezanian, N. (2014). Evaluation of diacetyl encapsulated alginate–whey protein microspheres release kinetics and mechanism at simulated mouth conditions. Food Research International, 56, 211-217. doi: