Increasing the Microbial and Oxidative Stability of Buffalo Meat using a Bioactive Edible Coating Based on Cordia myxa Fruit Mucilage and Citrus sinensis Eessential Oil

Document Type : Original Paper

Authors

1 Assistant Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

2 Associate Professor, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

3 Assistant Professor, Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

4 DVM, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

5 MSc. Student, Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran

Abstract

In this study, Citrus sinensis essential oil was extracted by hydrodistillation method and the results of gas chromatography-mass spectrometry showed that the oil is rich in limonene (87.86%). Total phenol and flavonoid contents of the CSEO were 125.41 mg GAE/g and 32.50 mg QE/g, respectively. Its antioxidant activity was found to be 39.75 and 52.43%, based on DPPH and ABTS radical scavenging activity, respectively. The presence of limonene in the CSEO was confirmed by furrier transform infrared spectroscopy. The CSEO was then combined with Cordia myxa fruit mucilage to produce an edible coating in order to improve the microbial and oxidative stability of buffalo meat during storage (10 days, 4 °C). Results showed that the growth of total viable count, psychrotrophic bacteria, E. coli, Staphylococcus aureus, and fungi were significantly inhibited in the buffalo meat samples coated with CMFM+CSEO. The CMFM+CSEO edible coating was also more effective in inhibiting the oxidation progression and moisture and texture losses, in comparison with control sample. According to the sensory evaluation results, the CMFM+CSEO edible coating increased the shelf-life of buffalo meat samples; the samples were acceptable even at the end of storage period (10 days), but the control sample was unacceptable after 7 days of storage. Therefore, the edible coating based on Cordia myxa fruit mucilage and Citrus sinensis essential oil could be used as a natural preservative to increase the shelf-life of buffalo meat and other meat products.

Keywords

Akolade, J. O., Nasir-Naeem, K. O., Swanepoel, A., Yusuf, A. A., Balogun, M., & Labuschagne, P. (2020). CO2-assisted production of polyethylene glycol / lauric acid microparticles for extended release of Citrus aurantifolia essential oil. Journal of CO2 Utilization, 38, 375-384. doi:https://doi.org/10.1016/j.jcou.2020.02.014
Alizadeh Behbahani, B., & Fooladi, A. A. I. (2018). Shirazi balangu (Lallemantia royleana) seed mucilage: Chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs. International Journal of Biological Macromolecules, 114, 882-889. doi:https://doi.org/10.1016/j.ijbiomac.2018.03.177
Alizadeh Behbahani, B., Noshad, M., & Falah, F. (2019). Cumin essential oil: Phytochemical analysis, antimicrobial activity and investigation of its mechanism of action through scanning electron microscopy. Microbial pathogenesis, 136, 103716. doi:https://doi.org/10.1016/j.micpath.2019.103716
Alizadeh Behbahani, B., & Shahidi, F. (2020). Evaluation of microbial, chemical and sensory characteristics of coated lamb with Scutellaria lateriflora seed mucilage in combination with Carum copticum essential oil to shelf life extension at refrigerated storage. Iranian Food Science and Technology Research Journal, 16(4), 383-394. doi:https://doi.org/10.22067/ifstrj.v16i4.82449 (in Persian)
Alizadeh Behbahani, B., Tabatabaei Yazdi, F., Shahidi, F., Mortazavi, S. A., & Mohebbi, M. (2017). Principle component analysis (PCA) for investigation of relationship between population dynamics of microbial pathogenesis, chemical and sensory characteristics in beef slices containing Tarragon essential oil. Microbial pathogenesis, 105, 37-50. doi:https://doi.org/10.1016/j.micpath.2017.02.013
Asjad, H. M. M., Akhtar, M. S., Bashir, S., Din, B., Gulzar, F., Khalid, R., & Asad, M. (2013). Phenol, flavonoid contents and antioxidant activity of six common citrus plants in Pakistan. Journal of Pharmaceutical and Cosmetic Sciences, 1(1), 1-5.
Barzegar, H., Alizadeh Behbahani, B., & Mehrnia, M. A. (2020). Quality retention and shelf life extension of fresh beef using Lepidium sativum seed mucilage-based edible coating containing Heracleum lasiopetalum essential oil: an experimental and modeling study. Food Science and Biotechnology, 29(5), 717-728. doi:https://doi.org/10.1007/s10068-019-00715-4
Bazargani-Gilani, B., Aliakbarlu, J., & Tajik, H. (2015). Effect of pomegranate juice dipping and chitosan coating enriched with Zataria multiflora Boiss essential oil on the shelf-life of chicken meat during refrigerated storage. Innovative food science & emerging technologies, 29, 280-287. doi:https://doi.org/10.1016/j.ifset.2015.04.007
Chaijan, M., Benjakul, S., Visessanguan, W., & Faustman, C. (2005). Changes of pigments and color in sardine (Sardinella gibbosa) and mackerel (Rastrelliger kanagurta) muscle during iced storage. Food Chemistry, 93(4), 607-617. doi:https://doi.org/10.1016/j.foodchem.2004.10.035
Choulitoudi, E., Ganiari, S., Tsironi, T., Ntzimani, A., Tsimogiannis, D., Taoukis, P., & Oreopoulou, V. (2017). Edible coating enriched with rosemary extracts to enhance oxidative and microbial stability of smoked eel fillets. Food packaging and shelf life, 12, 107-113. doi:https://doi.org/10.1016/j.fpsl.2017.04.009
Das, S., Vishakha, K., Banerjee, S., Mondal, S., & Ganguli, A. (2020). Sodium alginate-based edible coating containing nanoemulsion of Citrus sinensis essential oil eradicates planktonic and sessile cells of food-borne pathogens and increased quality attributes of tomatoes. International Journal of Biological Macromolecules, 162, 1770-1779. doi:https://doi.org/10.1016/j.ijbiomac.2020.08.086
Dehghan, B., Esmaeilzadeh Kenari, R., & Raftani Amiri, Z. (2019). Investigate the Antioxidant Properties of Orange Peel Essential Oil (Citrus sinesis) on the Stability of Soybean Oil During Storage Conditions. Journal of Food Technology and Nutrition, 16(3), 73-90. (in Persian)
Ebrahimi Hemmati Kaykha, M., Jooyandeh, H., Alizadeh Behbahani, B., & Noshad, M. (2020). Antimicrobial potential of Cordia myxa fruit on pathogenic bacteria: A study “in vitro” laboratory conditions. Food Science and Technology, 17(101), 71-80. (in Persian)
Galvão, J., Silva, V., Ferreira, S., França, F., Santos, D., Freitas, L., . . . Nunes, R. (2015). β-cyclodextrin inclusion complexes containing Citrus sinensis (L.) Osbeck essential oil: An alternative to control Aedes aegypti larvae. Thermochimica Acta, 608, 14-19. doi:https://doi.org/10.1016/j.tca.2015.04.001
Guerrero, A., Ferrero, S., Barahona, M., Boito, B., Lisbinski, E., Maggi, F., & Sañudo, C. (2020). Effects of active edible coating based on thyme and garlic essential oils on lamb meat shelf life after long-term frozen storage. Journal of the Science of Food and Agriculture, 100(2), 656-664. doi:https://doi.org/10.1002/jsfa.10061
Hassanzadeh, P., Tajik, H., RAZAVI, R. M., Ehsani, A., Aliakbarlu, J., & Moradi, M. (2012). Effects of gamma irradiation and chitosan edible coating on the bacterial, chemical and sensory properties of chicken meat. Journal of Food Research, 21(3), 355-370. (in Persian)
Heydari, S., Jooyandeh, H., Alizadeh Behbahani, B., & Noshad, M. (2020). The impact of Qodume Shirazi seed mucilage-based edible coating containing lavender essential oil on the quality enhancement and shelf life improvement of fresh ostrich meat: An experimental and modeling study. Food Science & Nutrition, 8(12), 6497-6512. doi:https://doi.org/10.1002/fsn3.1940
Joukar, F., Hosseini, S. M. H., Moosavi-Nasab, M., Mesbahi, G. R., & Behzadnia, A. (2017). Effect of Farsi gum-based antimicrobial adhesive coatings on the refrigeration shelf life of rainbow trout fillets. LWT, 80, 1-9. doi:https://doi.org/10.1016/j.lwt.2017.01.074
Kiarsi, Z., Hojjati, M., Behbahani, B. A., & Noshad, M. (2020). In vitro antimicrobial effects of Myristica fragrans essential oil on foodborne pathogens and its influence on beef quality during refrigerated storage. Journal of food safety, 40(3), e12782. doi:https://doi.org/10.1111/jfs.12782
Noshad, M., Alizadeh behbahani, B., & Dehghani, S. (2020). Improving oxidative and microbial stability of beef by using a bioactive edible coating obtained from Plantago lanceolata seed mucilage and loaded with Thymus vulgaris. Journal of food science and technology(Iran), 17(101), 1-13. (in Persian)
Noshad, M., Alizadeh Behbahani, B., Jooyandeh, H., Rahmati-Joneidabad, M., Hemmati Kaykha, M. E., & Ghodsi Sheikhjan, M. (2021). Utilization of Plantago major seed mucilage containing Citrus limon essential oil as an edible coating to improve shelf-life of buffalo meat under refrigeration conditions. Food Science & Nutrition, 9(3), 1625-1639. doi:https://doi.org/10.1002/fsn3.2137
Noshad, M., Hojjati, M., & Alizadeh Behbahani, B. (2020). Increasing Beef Shelf Life Using Bioactive Edible Coating Based on Dragon’s Head Seed Mucilage Loaded with Caraway Essential Oil. Iranian Journal of Biosystems Engineering, 51(2), 407-418. doi:https://doi.org/10.22059/ijbse.2020.299334.665289 (in Persian)
Noshad, M., Jooyandeh, H., Rahmati-Joneidabad, M., & Ghodsi Sheikhjan, M. (2021). In vitro investigation of the antimicrobial activity of Dezfuli orange peel essential oil with and without common antibiotics on some pathogenic bacteria. Food Science and Technology, 18(111), 159-167. (in Persian)
Nowzari, F., Shábanpour, B., & Ojagh, S. M. (2013). Comparison of chitosan–gelatin composite and bilayer coating and film effect on the quality of refrigerated rainbow trout. Food Chemistry, 141(3), 1667-1672. doi:https://doi.org/10.1016/j.foodchem.2013.03.022
Oriani, V. B., Molina, G., Chiumarelli, M., Pastore, G. M., & Hubinger, M. D. (2014). Properties of Cassava Starch-Based Edible Coating Containing Essential Oils. Journal of Food Science, 79(2), E189-E194. doi:https://doi.org/10.1111/1750-3841.12332
Pasbani, E., & Amiri, S. (2017). Evaluating the effect of aleo vera gel coating and solid lipid nano-particles containing thymol seed (Carum copticum) essential oil on shelf life of fresh beef. Iranian Journal of Nutrition Sciences & Food Technology, 12(2), 75-86. (in Persian)
Perini, J., Silvestre, W., Agostini, F., Toss, D., & Pauletti, G. (2017). Fractioning of orange (Citrus sinensis L.) essential oil using vacuum fractional distillation. Separation Science and Technology, 52(8), 1397-1403. doi:https://doi.org/10.1080/01496395.2017.1290108
Pirnia, M., Edalatian Dovom, M. R., Tabatabaee Yazdi, F., & Shahidi, F. (2015). The antibacterial effects of the aqueous and Ethanolic extracts of Cordiamyxa L. fruit on Staphylococcus aureus, Bacillus Cereus, Escherichia coli, and salmonella typhi. Qom University of Medical Sciences Journal, 9(4), 39-48. (in Persian)
Pirnia, M., Edalatian, M. R., TABATABAEE, Y. F., & Shahidi, F. (2016). Efficiency of response surface methodology to optimization ethanolic extract from cordia myxa and evaluation of antimicrobial activity of extract against a number of pathogenic microorganisms. Iranian Journal of Food Science and Technology, 13(55), 55-67. (in Persian)
Rossi, Y. E., & Palacios, S. M. (2013). Fumigant toxicity of Citrus sinensis essential oil on Musca domestica L. adults in the absence and presence of a P450 inhibitor. Acta tropica, 127(1), 33-37. doi:https://doi.org/10.1016/j.actatropica.2013.03.009
Sharma, N., & Tripathi, A. (2006). Fungitoxicity of the essential oil of Citrus sinensis on post-harvest pathogens. World Journal of Microbiology and Biotechnology, 22(6), 587-593. doi:https://doi.org/10.1007/s11274-005-9075-3
Sharma, N., & Tripathi, A. (2008). Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem. Microbiological research, 163(3), 337-344. doi:https://doi.org/10.1016/j.micres.2006.06.009
Singh, B., Singh, J. P., Kaur, A., & Yadav, M. P. (2021). Insights into the chemical composition and bioactivities of citrus peel essential oils. Food Research International, 143, 110231. doi:https://doi.org/10.1016/j.foodres.2021.110231
Tabatabaei Yazdi, F., Alizadeh Behbahani, B., Vasiee, A., Roshanak, S., & Mortazavi, A. (2017). Production of an antimicrobial edible coating based on Plantago major seed mucilage in combination with Heracleum persicum essential oil: its properties and application in beef. Microbiology in Food Industries, 3(3), 1-21. (in Persian)
Tanavar, H., Barzegar, H., Alizadeh Behbahani, B., & Mehrnia, M. A. (2020). Mentha pulegium essential oil: chemical composition, total phenolic and its cytotoxicity on cell line HT29. Iranian Journal Food Science and Technology Research, 16(5), 643-653. doi:https://doi.org/10.22067/ifstrj.v16i5.84722 (in Persian)
Tao, N. g., Liu, Y. j., & Zhang, M. l. (2009). Chemical composition and antimicrobial activities of essential oil from the peel of bingtang sweet orange (Citrus sinensis Osbeck). International journal of food science & technology, 44(7), 1281-1285. doi:https://doi.org/10.1111/j.1365-2621.2009.01947.x
Valipour Kootenaie, F., Ariaii, P., Khademi Shurmasti, D., & Nemati, M. (2017). Effect of chitosan edible coating enriched with eucalyptus essential oil and α‐tocopherol on silver carp fillets quality during refrigerated storage. Journal of food safety, 37(1), e12295. doi:https://doi.org/10.1111/jfs.12295
Vital, A. C. P., Guerrero, A., Kempinski, E. M. B. C., de Oliveira Monteschio, J., Sary, C., Ramos, T. R., . . . do Prado, I. N. (2018). Consumer profile and acceptability of cooked beef steaks with edible and active coating containing oregano and rosemary essential oils. Meat Science, 143, 153-158. doi:https://doi.org/10.1016/j.meatsci.2018.04.035
Youcef-Ettoumi, K., Zouambia, Y., & Moulai-Mostefa, N. (2021). Chemical composition, antimicrobial and antioxidant activities of Algerian Citrus sinensis essential oil extracted by hydrodistillation assisted by electromagnetic induction heating. Journal of Food Science and Technology, 58(8), 3049-3055. doi:https://doi.org/10.1007/s13197-020-04808-5
Zaidi, S., & Dahiya, P. (2015). In vitro antimicrobial activity, phytochemical analysis and total phenolic content of essential oil from Mentha spicata and Mentha piperita. International Food Research Journal, 22(6), 2440-2445.
CAPTCHA Image
Volume 10, Issue 2
September 2021
Pages 217-234
  • Receive Date: 05 May 2021
  • Revise Date: 14 August 2021
  • Accept Date: 08 September 2021