Investigating the effect of whey protein-starch conjugate on quality attributes of oil-in-water emulsion

Sadeghian, Ali Reza; Kadkhodaee, Rassoul; Farhoosh, Reza; Koocheki, Arash; Najaf Najafi, Masoud

doi:10.22101/JRIFST.2013.09.16.223

Investigating the effect of whey protein-starch conjugate on quality attributes of oil-in-water emulsion

Document Type : Original Paper

Authors

¹ PhD. Student, Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad

² Associate professor, Department of Food Nanotechnology, Research Institute of Food Science and Technology

³ Professor, Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad

⁴ Associate professor, Department of Food Science and Technology, College of Agriculture, Ferdowsi University of Mashhad

⁵ Assistant professor, Department of Food science and Technology, Institute of Scientific - Applied Higher Education Jihad-e-Agriculture

10.22101/JRIFST.2013.09.16.223

Abstract

Whey proteins are of wide application in the formation and stabilization of food emulsions. However, due to their high sensitivity to environmental conditions they quickly lose their functional properties. Glycolation of whey protein fractions is one of the methods used to modify their structure and improve their functional characteristics. The present work was carried out with the aim to prepare whey protein- starch conjugates and investigate the effect of protein to polysaccharide ratio on the quality attributes of oil-in-water emulsion. The results indicated that surface electrical charge decreased by increasing starch to protein ratio. On the other hand, changes in the surface and interfacial tension did not follow a clear trend in response to altering the mass ratio of protein. The drop size distribution curves were monomodal and nearly normal for all samples and became noticeably sharp with minimum width at protein to starch ratio 1:1. The finest and largest droplets were observed in the aforementioned sample and the one stabilized by modified starch, respectively. Studying the flow behavior of emulsions also revealed that starch containing sample exhibited the characteristics of near-Newtonian fluid, whilst the others were shown to have shear thinning pseudoplastic properties which became more distinct by increasing protein ratio leading to highest consistency index and lowest flow behavior index at %100 whey protein.

Keywords

References

Akhtar, M. & Dickinson, E. 2003. Emulsifying properties of whey protein-dextran conjugates at low pH and different salt concentrations. Colloids and Surfaces B: Biointerfaces, 31: 125-132.

Akhtar, M. & Dickinson, E. 2007. Whey protein-maltodextrin conjugates as emulsifying agents: an alternative to gum arabic. Food Hydrocolloids, 21: 607-616.

Baniel, A., Caer, D., Colas, B. & Gueguen, J. 1992. Functional properties of glycosylated derivatives of the 11S storage protein from pea (Pisum sativum L.). Journal of Agricultural and food Chemistry, 40: 200-205.

Benichou, A., Aserin, A., Lutz, R. & Garti, N. 2002. Formation and characterization of amphiphilic conjugates of whey protein isolate (WPI)/xanthan to improve surface activity. Food Hydrocolloids, 21: 379-391.

Corzo-Martı´nez, M., Moreno, F. J., Villamiel, M. & Harte, F. M. 2010. Characterization and improvement of rheological properties of sodium caseinate glycated with galactose, lactose and dextran. Food Hydrocolloids, 24: 88-97.

Dickinson, E. 2008. Hydrocolloids as emulsifiers and emulsion stabilizers. Food Hydrocolloids, 23: 1473-1482.

Dickinson, E. & Semenova, M. G. 1992. Emulsifying properties of covalent protein-dextran hybrids. Colloids and Surfaces, 64: 299-310.

Diftis, N. G., Biliaderis, C. G. & Kiosseoglou, V. D. 2005. Rheological properties and stability of model salad dressing emulsions prepared with a dry-heated soybean protein isolate–dextran mixture. Food Hydrocolloids, 19: 1025-1031.

Diftis, N. & Kiosseoglou, V. 2003. Improvement of emulsifying properties of soybean protein isolate by conjugation with carboxymethyl cellulose. Food Chemistry, 81: 1-6.

Diftis, N., Billiaderis, K. & Kiosseoglou, V. 2005. Rheological properties and stability of model salad dressing emulsions prepared with a dry-heated soybean protein isolate-dextran mixture. Food Hydrocolloids, 19: 1025-1031.

Einhorn-Stoll, U., Ulbrich, M., Sever, S. & Kunzek, H. 2005. Formation of milk proteinepectin conjugates with improved emulsification properties by controlled dry heating. Food Hydrocolloids, 19: 329-340.

Fechner, A., Knoth, A., Scherze, I. & Muschiolik, G. 2007. Stability and release properties of double-emulsions stabilized by caseinateedextran conjugates. Food Hydrocolloids, 21: 943-952.

Guo, X. & Xiong, Y. L. 2013. Characteristics and functional properties of buckwheat protein-sugar Schiff base complexes. LWT - Food Science and Technology, 51: 397-404.

Ibanoglu, E. & Ercelebi, E. A. 2007. Thermal denaturation and functional properties of egg proteins in the presence of hydrocolloid gums. Food Chemistry, 101: 626-633.

Kasran, M., Cui, S. W. & Goff, H. D. 2013. Covalent attachment of fenugreek gum to soy whey protein isolate through natural Maillard reaction for improved emulsion stability. Food Hydrocolloids, 30: 552-558.

Katsuda, M. S., McClements, D. J., Miglioranza, L. H. S. & Decker, E. A. 2008. Physical and oxidative stability of ﬁsh oil-in-water emulsions stabilized with β-lactoglobulin and pectin. Journal of Agricultural and Food Chemistry, 56: 5926–5931.

Kim, H. J., Decker, E. A. & McClements, D. J. 2002. Role of post-adsorption conformation of α-lactoglobulin on its ability to stabilize oil droplets against flocculation during heating at neutral pH. Langmuir, 18: 7577-7583.

Lesmes, U. & McClements, D. J. 2012. Controlling lipid digestibility: Response of lipid droplets coated by β-lactoglobulin-dextran maillard conjugates to stimulated gastrointestinal conditions. Food Hydrocolloids, 26: 221-230.

Li, Y., Lu, F., Luo, C. R., Chen, Z. X., Mao, J. & Shoemaker, C. 2009. Functional properties of the Maillard reaction products of rice protein with sugar. Food Chemistry, 117: 69-74.

Lin, L. H., Chen, K. M., Liu, H. J., Chu, H. C., Kuo, T. C., Hwang, M. C. & Wang, C. F. 2012. Preparation and surface activities of modified gelatin–glucose conjugates. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 408: 97-103.

Liu, G. & Zhong, Q. 2013. Thermal aggregation properties of whey protein glycated with various saccharides. Food Hydrocolloids, 32: 87-96.

Monahan, F. J., German, J. B. & Kinsella, J. E. 1995. Effect of pH and temperature on protein unfolding and thiol/disulfide interchange reactions during heat induced gelation of whey proteins. Journal of Agricultural and Food Chemistry, 43: 46-52.

Mu L., Zhao H., Zhao M., Cui Ch. & Liu L. 2011. Physicochemical properties of soy protein isolates- acacia gum conjugates. Czech Journal Food Science, 29: 129–136.

Nagasawa, K., Takahashi, K. & Hattori, M. 1996. Improved emulsifying properties of β- lactoglobulin by conjugating with carboxymethyl dextran. Food Hydrocolloids, 10: 63–67.

O’Regan, J. & Mulvihill, D. M. 2010. Heat stability and freeze-thaw stability of oil-in-water emulsions stabilized by sodium caseinate-maltodextrin conjugates. Food Chemistry, 119: 182-190.

Oliver, C. M., Melton, L. D., & Stanley, R. A. 2006. Creating proteins with novel functionality via the Maillard reaction: a review. Critical Reviews in Food Science and Nutrition, 46: 337–350.

Paraman, I., Hettiarachchy, N. S. & Schaefer, C. 2007. Glycosylation and deamidation of rice endosperm protein for improved solubility and emulsifying properties. Cereal Chemistry, 84: 593–599.

Sun, C., Gunasekaran, S. & Richards, M. P. 2007. Effect of xanthan gum on physicochemical properties of whey protein isolate stabilized oil-in-water emulsions. Food Hydrocolloids, 21: 555-564.

Sun, Y. X., Hayakawa, S., Puangmanee, S. & Izumori, K. 2006. Chemical properties and antioxidative activity of glycated α-lactalbumin with a rare sugar, D-allose, by Maillard reaction. Food Chemistry, 95: 509-517.

Turgeon, S. L., Beaulieu, M., Schmitt, C. & Sanchez, C. 2003. Protein–polysaccharide interactions: phase-ordering kinetics, thermodynamic and structural aspects. Current Opinion in Colloid and Interface Science, 8: 401–414.

Wooster, T. J. & Augustin, M. A. 2006. β-Lactoglobulin-dextran Maillard conjugates: Their effect on interfacial thickness and emulsion stability. Journal of Colloid and Interface Science, 303: 564-572.

Xu, D., Wang, X., Jiang, J., Yuan, F. & Gao, Y. 2012. Impact of whey protein e Beet pectin conjugation on the physicochemical stability of β-carotene emulsions. Food Hydrocolloids, 28: 258-266.