Evaluation of pistachio aflatoxin (B1) detection by raman and surface enhanced raman spectroscopy (SERS) using two substrates composed of silver nanoparticles

Mohammadigol, Reza; Malekfar, Rasoul; Mirabolfathi, Mansoureh; Behmadi, Homa

doi:10.22101/JRIFST.2015.07.23.422

Evaluation of pistachio aflatoxin (B1) detection by raman and surface enhanced raman spectroscopy (SERS) using two substrates composed of silver nanoparticles

Document Type : Original Paper

Authors

¹ Assistant Professor, Department of Biosystem Mechanics Engineering, Faculty of Agriculture, Arak University, Arak, Iran

² Professor, Department of Atomic Physics and Molecular, , Faculty of Basic sciences, Tarbiat Modares University, Tehran, Iran

³ Professor, Department of Plant Disease, Iranian Research Institute of Plant Protection, Tehran, Iran

⁴ Department of Food Science, Agricultural Engineering Research Institute, Karaj, Iran

10.22101/JRIFST.2015.07.23.422

Abstract

In this study, the feasibility of detection of pistachio aflatoxin (B1) using Raman spectroscopy and Surface Enhanced Raman Spectroscopy (SERS) was investigated. for this purpose, the pistachio samples were used in paste form and infected with B₁ aflatoxin in concentration of 5 ppb. Raman spectra of healthy and infected pistachio samples were compared. In order to detect aflatoxin B₁, the SERS was applied to two types of substrates: Ag nanowires and Ag nanoparticles colloid on glass. The results showed that the detection of 5 ppb concentration of aflatoxin B₁ was not feasible. Between the two studied substrates, Ag nanoparticles colloid showed better results to detect aflatoxin B₁. Comparing the corresponding peaks of wave numbers of the aflatoxin B₁ Raman spectrum (1000 ppb) to the values obtained from computational method (DFT), peaks in 945, 1048, 1148, 1252, 1390, 1527, 1583, 1658 cm^-1 were confirmed as the fingerprints of aflatoxin B₁ Raman spectrum. We conclude that the Raman spectroscopy has the potential to detect the infection of the aflatoxin B₁ in 1000 ppb concentration.

Keywords

References

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