Beta-Carotene loaded nanoliposome: effects of gama –oryzanol on particle size stability and encapsulation

Document Type : Original Paper


1 MSc. Graduated Student, Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Professor, Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

3 Associated Professor, Department of Pharmaceutical Sciences, Drug Applied Research Center, Tabriz University of Medical Sciences, Iran

4 Associated Professor, Department of Food Science and Technology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran


Beta-carotene is one of the most important types of functional compounds, antioxidant and precursor of vitamin A which can be find in plant based products. The enrichment of food with beta carotene is difficult due to its hydrophobic nature and low stability. The encapsulation of β-carotene in a lipid carrier such as liposome is a method which potentially can reduce these problems. In this research, the ß-carotene bearing liposomal system   prepared using modified thermal method and Gama-oryzanol, as a phytosterols, was used to increase the stability of the liposome structure. Intending to ensure the capsulation of beta carotene in liposomes, functional groups and possible interaction between beta-carotene and lecithin was examined by infrared spectroscopy (FTIR) and peaks of 980 and 1580 cm-1 corresponding to the functional β-carotene, were observed in liposomes. Not only the particle size was obtained around 64 nm at different ratio of lecithin to β-carotene, but also it was below 500 nm during 30 days of storage time.  Encapsulation efficiency of β-carotene at high concentrations of lecithin was % 77.25 and it declined to the %69.73 during storage. Using Gama-oryzanol in β-carotene bearing liposome increased the stability of particle size and encapsulation during the storage period.


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Volume 4, Issue 4
January 2016
Pages 365-382
  • Receive Date: 05 January 2015
  • Revise Date: 24 September 2015
  • Accept Date: 02 October 2015